LS-B10406

antibody from LSBio

Targeting: LAMP1 CD107a

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-B10406

Provider

LSBio

Product name

IHC-plus™ LAMP1 / CD107a Antibody (aa32-350, clone 5H6) LS-B10406

Antibody type

Monoclonal

Description

Affinity purified

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

5H6

Storage

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western strip blots of HeLa cell crude extracts stained with anti LAMP1 antibody CD107a / LAMP1 antibody in strip 9. Lane 10 shows staining another LAMP1 antibody. Both antibodies bind to a diffuse band running at between ~90 and ~120kDa as expected, and show no appreciably cross reactivity with any other protein.

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

HeLa cells staining with CD107a / LAMP1 antibody (red), and counterstained with chicken polyclonal antibody to Vimentin (green) and DNA (blue). The CD107a / LAMP1 antibody antibody reveals strong punctate cytoplasmic staining corresponding to lysosomes and late endosomes, while the Vimentin antibody reveals cytoplasmic intermediate filaments.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

HeLa cells staining with CD107a / LAMP1 antibody (red), and counterstained with chicken polyclonal antibody to Vimentin (green) and DNA (blue). The CD107a / LAMP1 antibody antibody reveals strong punctate cytoplasmic staining corresponding to lysosomes and late endosomes, while the Vimentin antibody reveals cytoplasmic intermediate filaments.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

HeLa cells staining with CD107a / LAMP1 antibody (red), and counterstained with chicken polyclonal antibody to Vimentin (green) and DNA (blue). The CD107a / LAMP1 antibody antibody reveals strong punctate cytoplasmic staining corresponding to lysosomes and late endosomes, while the Vimentin antibody reveals cytoplasmic intermediate filaments.