LS-B10407

antibody from LSBio

Targeting: LAMP1 CD107a

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-B10407

Provider

LSBio

Product name

IHC-plus™ LAMP1 / CD107a Antibody (clone 6E2) LS-B10407

Antibody type

Monoclonal

Description

Affinity purified

Reactivity

Human, Mouse, Rat, Bovine, Porcine

Host

Mouse

Isotype

IgG

Antibody clone number

6E2

Storage

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western strip blots of HeLa cell crude extracts with two different preparations of CD107a / LAMP1 antibody in strips 17 and 18. Both preparations bind to a diffuse band running at between ~90kDa and ~120kDa as expected, and show no appreciably cross reactivity with any other protein.

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

HeLa cells staining with CD107a / LAMP1 antibody (green), and counterstained with chicken polyclonal antibody to Lamin A/C (red) and DNA (blue). The CD107a / LAMP1 antibody antibody reveals strong cytoplasmic staining of lysosomes and early endosomes, while the Lamin A/C antibody binds to the nuclear lamina. Since both DNA (blue) and Lamin A/C (red) are associated with the nuclear compartment, this region appears crimson in this image.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

HeLa cells staining with CD107a / LAMP1 antibody (green), and counterstained with chicken polyclonal antibody to Lamin A/C (red) and DNA (blue). The CD107a / LAMP1 antibody antibody reveals strong cytoplasmic staining of lysosomes and early endosomes, while the Lamin A/C antibody binds to the nuclear lamina. Since both DNA (blue) and Lamin A/C (red) are associated with the nuclear compartment, this region appears crimson in this image.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

HeLa cells staining with CD107a / LAMP1 antibody (green), and counterstained with chicken polyclonal antibody to Lamin A/C (red) and DNA (blue). The CD107a / LAMP1 antibody antibody reveals strong cytoplasmic staining of lysosomes and early endosomes, while the Lamin A/C antibody binds to the nuclear lamina. Since both DNA (blue) and Lamin A/C (red) are associated with the nuclear compartment, this region appears crimson in this image.