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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- ABIN1580432 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Lysosomal-Associated Membrane Protein 1 (LAMP1) antibody
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- affinity purified antibody
- Reactivity
- Human, Mouse, Rat, Bovine, Porcine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 6E2
- Vial size
- 100 μL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term or -20°C long term.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Derived protein sequence, oligosaccharides, and membrane insertion of the 120-kDa lysosomal membrane glycoprotein (lgp120): identification of a highly conserved family of lysosomal membrane glycoproteins.
Translocation and clustering of endosomes and lysosomes depends on microtubules.
Howe CL, Granger BL, Hull M, Green SA, Gabel CA, Helenius A, Mellman I
Proceedings of the National Academy of Sciences of the United States of America 1988 Oct;85(20):7577-81
Proceedings of the National Academy of Sciences of the United States of America 1988 Oct;85(20):7577-81
Translocation and clustering of endosomes and lysosomes depends on microtubules.
Matteoni R, Kreis TE
The Journal of cell biology 1987 Sep;105(3):1253-65
The Journal of cell biology 1987 Sep;105(3):1253-65
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western strip blots of HeLa cell crude extracts with two different preparations of ABIN1580432 in strips 17 and 18. Both preparations bind to a diffuse band running at between ~90 kDa and ~120 kDa as expected, and show no appreciably cross reactivity with any other protein.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- HeLa cells staining with ABIN1580432 (green), and counterstained with ?s chicken polyclonal antibody to Lamin A/C, CPCA-LaminAC (red) and DNA (blue). The ABIN1580432 antibody reveals strong cytoplasmic staining of lysosomes and early endosomes, while the Lamin A/C antibody binds to the nuclear lamina. Since both DNA (blue) and Lamin A/C (red) are associated with the nuclear compartment, this region appears crimson in this image.
