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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- AP1823a - Provider product page
- Provider
- Abcepta
- Proper citation
- Abgent Cat#AP1823a, RRID:AB_2296835
- Product name
- LAMP1 Antibody (N-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references A rapid method to improve protein detection by indirect ELISA.
Granulovacuolar degeneration (GVD) bodies of Alzheimer's disease (AD) resemble late-stage autophagic organelles.
The Rac1/MKK7/JNK pathway signals upregulation of Atg5 and subsequent autophagic cell death in response to oncogenic Ras.
Hnasko R, Lin A, McGarvey JA, Stanker LH
Biochemical and biophysical research communications 2011 Jul 15;410(4):726-31
Biochemical and biophysical research communications 2011 Jul 15;410(4):726-31
Granulovacuolar degeneration (GVD) bodies of Alzheimer's disease (AD) resemble late-stage autophagic organelles.
Funk KE, Mrak RE, Kuret J
Neuropathology and applied neurobiology 2011 Apr;37(3):295-306
Neuropathology and applied neurobiology 2011 Apr;37(3):295-306
The Rac1/MKK7/JNK pathway signals upregulation of Atg5 and subsequent autophagic cell death in response to oncogenic Ras.
Byun JY, Yoon CH, An S, Park IC, Kang CM, Kim MJ, Lee SJ
Carcinogenesis 2009 Nov;30(11):1880-8
Carcinogenesis 2009 Nov;30(11):1880-8
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Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Western blot analysis of lysates from Hela,HT1080 cell line and human placenta tissue (from left to right),using LAMP1 Antibody (N-term)(Cat. #AP1823a).AP1823a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane."
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Fluorescent image of U251 cells stained with LAMP1 (N-term) antibody. U251 cells were treated with Chloroquine (50 ¼M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1823a LAMP1 (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ¼g/ml, 5 min). LAMP1 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells."
- Primary Ab dilution
- 1:200
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with *LAMP1 antibody (N-term) (Cat.#AP1823a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."
- Primary Ab dilution
- 1:10~50
