TA324642

antibody from OriGene

Targeting: LAMP1 CD107a

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA324642

Provider

OriGene

Product name

Rabbit polyclonal LAMP1 Antibody (N-term)

Antibody type

Polyclonal

Description

Rabbit polyclonal LAMP1 Antibody (N-term)

Host

Rabbit

Conjugate

Unconjugated

Epitope

LAMP1

Isotype

IgG

Antibody clone number

NULL

Vial size

400 µl

Concentration

0.5 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of lysates from Hela,HT1080 cell line and human placenta tissue (from left to right),using LAMP1 Antibody (N-term)(Cat. #TA324642).TA324642 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

IF image of U251 cells stained with LAMP1 (N-term) antibody. U251 cells were treated with Chloroquine , then incubated with TA324642 LAMP1 (N-term) primary antibody (1:200, 2 h at RT). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) . LAMP1 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with *LAMP1 antibody (N-term) (Cat.#TA324642), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Validation comment

IHC