Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-25183AF647 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal LAMP-1/CD107a Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified. The mouse monoclonal antibody H4A3 recognizes CD107a, an approximately 100-120 kDa glycoprotein expressed mainly on lysosomal, but also on the plasma membrane.
- Reactivity
- Human, Mouse, Simian
- Host
- Mouse
- Conjugate
- Red dye
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C in the dark.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) [Alexa Fluor 647] [NBP2-25183AF647] - An intracellular stain was performed on A549 cells with LAMP-1/CD107a antibody (H4A3) NBP2-25183AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.