Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- MAB4320 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse LAMP1/CD107a Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse LAMP1/CD107a in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human LAMP1/CD107a is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P11438
- Isotype
- IgG
- Antibody clone number
- 747203
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Amino Acid Transporter Slc38a5 Controls Glucagon Receptor Inhibition-Induced Pancreatic α Cell Hyperplasia in Mice.
Sphingosine regulates the NLRP3-inflammasome and IL-1β release from macrophages.
Kim J, Okamoto H, Huang Z, Anguiano G, Chen S, Liu Q, Cavino K, Xin Y, Na E, Hamid R, Lee J, Zambrowicz B, Unger R, Murphy AJ, Xu Y, Yancopoulos GD, Li WH, Gromada J
Cell metabolism 2017 Jun 6;25(6):1348-1361.e8
Cell metabolism 2017 Jun 6;25(6):1348-1361.e8
Sphingosine regulates the NLRP3-inflammasome and IL-1β release from macrophages.
Luheshi NM, Giles JA, Lopez-Castejon G, Brough D
European journal of immunology 2012 Mar;42(3):716-25
European journal of immunology 2012 Mar;42(3):716-25
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- LAMP1/CD107a in RAW 264.7 Mouse Cell Line. LAMP1/CD107a was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Rat Anti-Mouse LAMP1/CD107a Monoclonal Antibody (Catalog # MAB4320) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.