Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 25-1079-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), PE-Cyanine7, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBioH4A3 monoclonal antibody reacts with human CD107a, also known as lysosomal-associated membrane protein-1 (LAMP-1). CD107a is a highly glycosylated protein of approximately 110kDa. It is predominantly expressed intracellularly in the lysosomal/endosomal membrane in nearly all cells. CD107a is transiently expressed on the cell surface of degranulating cytolytic T cells, and is also upregulated on the surface of activated platelets and some cancer cells. Applications Reported: This eBioH4A3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This eBioH4A3 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of Jurkat cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- eBioH4A3
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Complement receptor CD46 co-stimulates optimal human CD8+ T cell effector function via fatty acid metabolism.
T cells targeting NY-ESO-1 demonstrate efficacy against disseminated neuroblastoma.
Inhibition of CD39 enzymatic function at the surface of tumor cells alleviates their immunosuppressive activity.
The small chemical vacuolin-1 alters the morphology of lysosomes without inhibiting Ca2+-regulated exocytosis.
Detection of T-cell degranulation: CD107a and b.
LAMP-1 and LAMP-2, but not LAMP-3, are reliable markers for activation-induced secretion of human mast cells.
Expression of Lamp-1 and Lamp-2 and their interactions with galectin-3 in human tumor cells.
Arbore G, West EE, Rahman J, Le Friec G, Niyonzima N, Pirooznia M, Tunc I, Pavlidis P, Powell N, Li Y, Liu P, Servais A, Couzi L, Fremeaux-Bacchi V, Placais L, Ferraro A, Walsh PR, Kavanagh D, Afzali B, Lavender P, Lachmann HJ, Kemper C
Nature communications 2018 Oct 10;9(1):4186
Nature communications 2018 Oct 10;9(1):4186
T cells targeting NY-ESO-1 demonstrate efficacy against disseminated neuroblastoma.
Singh N, Kulikovskaya I, Barrett DM, Binder-Scholl G, Jakobsen B, Martinez D, Pawel B, June CH, Kalos MD, Grupp SA
Oncoimmunology 2016;5(1):e1040216
Oncoimmunology 2016;5(1):e1040216
Inhibition of CD39 enzymatic function at the surface of tumor cells alleviates their immunosuppressive activity.
Bastid J, Regairaz A, Bonnefoy N, Déjou C, Giustiniani J, Laheurte C, Cochaud S, Laprevotte E, Funck-Brentano E, Hemon P, Gros L, Bec N, Larroque C, Alberici G, Bensussan A, Eliaou JF
Cancer immunology research 2015 Mar;3(3):254-65
Cancer immunology research 2015 Mar;3(3):254-65
The small chemical vacuolin-1 alters the morphology of lysosomes without inhibiting Ca2+-regulated exocytosis.
Huynh C, Andrews NW
EMBO reports 2005 Sep;6(9):843-7
EMBO reports 2005 Sep;6(9):843-7
Detection of T-cell degranulation: CD107a and b.
Betts MR, Koup RA
Methods in cell biology 2004;75:497-512
Methods in cell biology 2004;75:497-512
LAMP-1 and LAMP-2, but not LAMP-3, are reliable markers for activation-induced secretion of human mast cells.
Grützkau A, Smorodchenko A, Lippert U, Kirchhof L, Artuc M, Henz BM
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2004 Sep;61(1):62-8
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2004 Sep;61(1):62-8
Expression of Lamp-1 and Lamp-2 and their interactions with galectin-3 in human tumor cells.
Sarafian V, Jadot M, Foidart JM, Letesson JJ, Van den Brûle F, Castronovo V, Wattiaux R, Coninck SW
International journal of cancer 1998 Jan 5;75(1):105-11
International journal of cancer 1998 Jan 5;75(1):105-11
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of Jurkat cells with Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (blue histogram) or Anti-Human CD107a (LAMP-1) PE-Cyanine7 (purple histogram). Total cells were used for analysis.