Antibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 48-1079-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), eFluor 450, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBioH4A3 monoclonal antibody reacts with human CD107a, also known as lysosomal-associated membrane protein-1 (LAMP-1). CD107a is a highly glycosylated protein of approximately 110kDa. It is predominantly expressed intracellularly in the lysosomal/endosomal membrane in nearly all cells. CD107a is transiently expressed on the cell surface of degranulating cytolytic T cells, and is also upregulated on the surface of activated platelets and some cancer cells. Applications Reported: This eBioH4A3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This eBioH4A3 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of Jurkat cells using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- eBioH4A3
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Complement receptor CD46 co-stimulates optimal human CD8+ T cell effector function via fatty acid metabolism.
PD1 is expressed on exhausted T cells as well as virus specific memory CD8+ T cells in the bone marrow of myeloma patients.
T cells targeting NY-ESO-1 demonstrate efficacy against disseminated neuroblastoma.
Targeting cancer metabolism by simultaneously disrupting parallel nutrient access pathways.
Fucosylation of LAMP-1 and LAMP-2 by FUT1 correlates with lysosomal positioning and autophagic flux of breast cancer cells.
Munc18-2 is required for Syntaxin 11 Localization on the Plasma Membrane in Cytotoxic T-Lymphocytes.
The small chemical vacuolin-1 alters the morphology of lysosomes without inhibiting Ca2+-regulated exocytosis.
Detection of T-cell degranulation: CD107a and b.
LAMP-1 and LAMP-2, but not LAMP-3, are reliable markers for activation-induced secretion of human mast cells.
Expression of Lamp-1 and Lamp-2 and their interactions with galectin-3 in human tumor cells.
Isolation and characterization of human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2. Major sialoglycoproteins carrying polylactosaminoglycan.
Arbore G, West EE, Rahman J, Le Friec G, Niyonzima N, Pirooznia M, Tunc I, Pavlidis P, Powell N, Li Y, Liu P, Servais A, Couzi L, Fremeaux-Bacchi V, Placais L, Ferraro A, Walsh PR, Kavanagh D, Afzali B, Lavender P, Lachmann HJ, Kemper C
Nature communications 2018 Oct 10;9(1):4186
Nature communications 2018 Oct 10;9(1):4186
PD1 is expressed on exhausted T cells as well as virus specific memory CD8+ T cells in the bone marrow of myeloma patients.
Sponaas AM, Yang R, Rustad EH, Standal T, Thoresen AS, Dao Vo C, Waage A, Slørdahl TS, Børset M, Sundan A
Oncotarget 2018 Aug 10;9(62):32024-32035
Oncotarget 2018 Aug 10;9(62):32024-32035
T cells targeting NY-ESO-1 demonstrate efficacy against disseminated neuroblastoma.
Singh N, Kulikovskaya I, Barrett DM, Binder-Scholl G, Jakobsen B, Martinez D, Pawel B, June CH, Kalos MD, Grupp SA
Oncoimmunology 2016;5(1):e1040216
Oncoimmunology 2016;5(1):e1040216
Targeting cancer metabolism by simultaneously disrupting parallel nutrient access pathways.
Kim SM, Roy SG, Chen B, Nguyen TM, McMonigle RJ, McCracken AN, Zhang Y, Kofuji S, Hou J, Selwan E, Finicle BT, Nguyen TT, Ravi A, Ramirez MU, Wiher T, Guenther GG, Kono M, Sasaki AT, Weisman LS, Potma EO, Tromberg BJ, Edwards RA, Hanessian S, Edinger AL
The Journal of clinical investigation 2016 Nov 1;126(11):4088-4102
The Journal of clinical investigation 2016 Nov 1;126(11):4088-4102
Fucosylation of LAMP-1 and LAMP-2 by FUT1 correlates with lysosomal positioning and autophagic flux of breast cancer cells.
Tan KP, Ho MY, Cho HC, Yu J, Hung JT, Yu AL
Cell death & disease 2016 Aug 25;7(8):e2347
Cell death & disease 2016 Aug 25;7(8):e2347
Munc18-2 is required for Syntaxin 11 Localization on the Plasma Membrane in Cytotoxic T-Lymphocytes.
Dieckmann NM, Hackmann Y, Aricò M, Griffiths GM
Traffic (Copenhagen, Denmark) 2015 Dec;16(12):1330-41
Traffic (Copenhagen, Denmark) 2015 Dec;16(12):1330-41
The small chemical vacuolin-1 alters the morphology of lysosomes without inhibiting Ca2+-regulated exocytosis.
Huynh C, Andrews NW
EMBO reports 2005 Sep;6(9):843-7
EMBO reports 2005 Sep;6(9):843-7
Detection of T-cell degranulation: CD107a and b.
Betts MR, Koup RA
Methods in cell biology 2004;75:497-512
Methods in cell biology 2004;75:497-512
LAMP-1 and LAMP-2, but not LAMP-3, are reliable markers for activation-induced secretion of human mast cells.
Grützkau A, Smorodchenko A, Lippert U, Kirchhof L, Artuc M, Henz BM
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2004 Sep;61(1):62-8
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2004 Sep;61(1):62-8
Expression of Lamp-1 and Lamp-2 and their interactions with galectin-3 in human tumor cells.
Sarafian V, Jadot M, Foidart JM, Letesson JJ, Van den Brûle F, Castronovo V, Wattiaux R, Coninck SW
International journal of cancer 1998 Jan 5;75(1):105-11
International journal of cancer 1998 Jan 5;75(1):105-11
Isolation and characterization of human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2. Major sialoglycoproteins carrying polylactosaminoglycan.
Carlsson SR, Roth J, Piller F, Fukuda M
The Journal of biological chemistry 1988 Dec 15;263(35):18911-9
The Journal of biological chemistry 1988 Dec 15;263(35):18911-9
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Intracellular staining of Jurkat cells with Mouse IgG1 K Isotype Control eFluor® 450 (Product # 48-4714-82) (blue histogram) or Anti-Human CD107a (LAMP-1) eFluor® 450 (purple histogram) using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Total viable cells, as determined by Fixable Viability Dye eFluor® 660 (Product # 65-0864-14), were used for analysis.