Antibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- 53-1079-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), Alexa Fluor 488, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBioH4A3 monoclonal antibody reacts with human CD107a, also known as lysosomal-associated membrane protein-1 (LAMP-1). CD107a is a highly glycosylated protein of approximately 110kDa. It is predominantly expressed intracellularly in the lysosomal/endosomal membrane in nearly all cells. CD107a is transiently expressed on the cell surface of degranulating cytolytic T cells, and is also upregulated on the surface of activated platelets and some cancer cells. Applications Reported: This eBioH4A3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. It has also been reported for use in surface staining in a flow cytometric based degranulation assay. Applications Tested: This eBioH4A3 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- eBioH4A3
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Complement receptor CD46 co-stimulates optimal human CD8+ T cell effector function via fatty acid metabolism.
Broad CD8+ T cell cross-recognition of distinct influenza A strains in humans.
T cells targeting NY-ESO-1 demonstrate efficacy against disseminated neuroblastoma.
Targeting cancer metabolism by simultaneously disrupting parallel nutrient access pathways.
Fucosylation of LAMP-1 and LAMP-2 by FUT1 correlates with lysosomal positioning and autophagic flux of breast cancer cells.
Munc18-2 is required for Syntaxin 11 Localization on the Plasma Membrane in Cytotoxic T-Lymphocytes.
Serum IgE clearance is facilitated by human FcεRI internalization.
Both plasmacytoid dendritic cells and monocytes stimulate natural killer cells early during human herpes simplex virus type 1 infections.
Merkel cell polyomavirus large T antigen disrupts lysosome clustering by translocating human Vam6p from the cytoplasm to the nucleus.
Biologic and immunologic effects of preoperative trastuzumab for ductal carcinoma in situ of the breast.
The small chemical vacuolin-1 alters the morphology of lysosomes without inhibiting Ca2+-regulated exocytosis.
Arbore G, West EE, Rahman J, Le Friec G, Niyonzima N, Pirooznia M, Tunc I, Pavlidis P, Powell N, Li Y, Liu P, Servais A, Couzi L, Fremeaux-Bacchi V, Placais L, Ferraro A, Walsh PR, Kavanagh D, Afzali B, Lavender P, Lachmann HJ, Kemper C
Nature communications 2018 Oct 10;9(1):4186
Nature communications 2018 Oct 10;9(1):4186
Broad CD8+ T cell cross-recognition of distinct influenza A strains in humans.
Grant EJ, Josephs TM, Loh L, Clemens EB, Sant S, Bharadwaj M, Chen W, Rossjohn J, Gras S, Kedzierska K
Nature communications 2018 Dec 21;9(1):5427
Nature communications 2018 Dec 21;9(1):5427
T cells targeting NY-ESO-1 demonstrate efficacy against disseminated neuroblastoma.
Singh N, Kulikovskaya I, Barrett DM, Binder-Scholl G, Jakobsen B, Martinez D, Pawel B, June CH, Kalos MD, Grupp SA
Oncoimmunology 2016;5(1):e1040216
Oncoimmunology 2016;5(1):e1040216
Targeting cancer metabolism by simultaneously disrupting parallel nutrient access pathways.
Kim SM, Roy SG, Chen B, Nguyen TM, McMonigle RJ, McCracken AN, Zhang Y, Kofuji S, Hou J, Selwan E, Finicle BT, Nguyen TT, Ravi A, Ramirez MU, Wiher T, Guenther GG, Kono M, Sasaki AT, Weisman LS, Potma EO, Tromberg BJ, Edwards RA, Hanessian S, Edinger AL
The Journal of clinical investigation 2016 Nov 1;126(11):4088-4102
The Journal of clinical investigation 2016 Nov 1;126(11):4088-4102
Fucosylation of LAMP-1 and LAMP-2 by FUT1 correlates with lysosomal positioning and autophagic flux of breast cancer cells.
Tan KP, Ho MY, Cho HC, Yu J, Hung JT, Yu AL
Cell death & disease 2016 Aug 25;7(8):e2347
Cell death & disease 2016 Aug 25;7(8):e2347
Munc18-2 is required for Syntaxin 11 Localization on the Plasma Membrane in Cytotoxic T-Lymphocytes.
Dieckmann NM, Hackmann Y, Aricò M, Griffiths GM
Traffic (Copenhagen, Denmark) 2015 Dec;16(12):1330-41
Traffic (Copenhagen, Denmark) 2015 Dec;16(12):1330-41
Serum IgE clearance is facilitated by human FcεRI internalization.
Greer AM, Wu N, Putnam AL, Woodruff PG, Wolters P, Kinet JP, Shin JS
The Journal of clinical investigation 2014 Mar;124(3):1187-98
The Journal of clinical investigation 2014 Mar;124(3):1187-98
Both plasmacytoid dendritic cells and monocytes stimulate natural killer cells early during human herpes simplex virus type 1 infections.
Vogel K, Thomann S, Vogel B, Schuster P, Schmidt B
Immunology 2014 Dec;143(4):588-600
Immunology 2014 Dec;143(4):588-600
Merkel cell polyomavirus large T antigen disrupts lysosome clustering by translocating human Vam6p from the cytoplasm to the nucleus.
Liu X, Hein J, Richardson SC, Basse PH, Toptan T, Moore PS, Gjoerup OV, Chang Y
The Journal of biological chemistry 2011 May 13;286(19):17079-90
The Journal of biological chemistry 2011 May 13;286(19):17079-90
Biologic and immunologic effects of preoperative trastuzumab for ductal carcinoma in situ of the breast.
Kuerer HM, Buzdar AU, Mittendorf EA, Esteva FJ, Lucci A, Vence LM, Radvanyi L, Meric-Bernstam F, Hunt KK, Symmans WF
Cancer 2011 Jan 1;117(1):39-47
Cancer 2011 Jan 1;117(1):39-47
The small chemical vacuolin-1 alters the morphology of lysosomes without inhibiting Ca2+-regulated exocytosis.
Huynh C, Andrews NW
EMBO reports 2005 Sep;6(9):843-7
EMBO reports 2005 Sep;6(9):843-7
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Enhanced validation
- Submitted by
- Invitrogen Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Knockdown of CD107a (LAMP1) was achieved by transfecting HeLa cells with CD107a (LAMP1) specific siRNAs (Silencer®
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Left: 3-day PHA-stimulated normal human peripheral blood cells were surface stained with 0.125 µg of Anti-Human CD107a (LAMP-1) Alexa Fluor® 488. Total viable cells were used for analysis. Right: Normal human peripheral blood cells were stained intracellularly with 0.125 µg of Anti-Human CD107a (LAMP-1) Alexa Fluor® 488. Total cells were used for analysis.
- Conjugate
- Green dye