- LS-C783540 - LSBio LAMP1 antibody

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Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Western blot of purified anti-LAMP-1 antibody (clone H4A3). Lane 1: Molecular weight marker; Lane 2: 20 µg of Hela cell lysate; Lane 3: 20 µg of NIH3T3 cell lysate; Lane 4: 20 µg of human brain lysate; Lane 5: 20 µg of mouse brain lysate; Lane 6: 10 ng of human recombinant LAMP-1. The blot was incubated with 1 µg/ml of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG. Anti-ß-actin antibody was used as the loading control. Enhanced chemiluminescence was used as the detection system.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: ICC staining of purified anti-LAMP-1 antibody (clone H4A3) on Hela cells. The cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then stained with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-Mouse IgG for one hour at room temperature. Cells were counterstained with Phalloidin™ Green 488 and DAPI to visualize actin filaments and nuclei, respectively. The images were captured with a 60X objective. Scale bar: 50 µm.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: ICC staining of purified anti-LAMP-1 antibody (clone H4A3) on Hela cells. The cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then stained with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-Mouse IgG for one hour at room temperature. Cells were counterstained with Phalloidin™ Green 488 and DAPI to visualize actin filaments and nuclei, respectively. The images were captured with a 60X objective. Scale bar: 50 µm.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: ICC staining of purified anti-LAMP-1 antibody (clone H4A3) on Hela cells. The cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then stained with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-Mouse IgG for one hour at room temperature. Cells were counterstained with Phalloidin™ Green 488 and DAPI to visualize actin filaments and nuclei, respectively. The images were captured with a 60X objective. Scale bar: 50 µm.

LS-C783540