MA5-29385

antibody from Invitrogen Antibodies

Targeting: LAMP1 CD107a

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-29385

Provider

Invitrogen Antibodies

Product name

LAMP1 Recombinant Rabbit Monoclonal Antibody (107)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire. This antibody has specificity for Human LAMP1/CD107a.

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

107

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Application of meso-CF(3)-Fluorophore BODIPY with Phenyl and Pyrazolyl Substituents for Lifetime Visualization of Lysosomes.

Trukhan IS, Tomilin DN, Dremina NN, Sobenina LN, Shurygin MG, Petrushenko KB, Petrushenko IK, Trofimov BA, Shurygina IA
Molecules (Basel, Switzerland) 2022 Aug 7;27(15)

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Confocal immunofluorescence analysis of Human LAMP1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with LAMP1 Recombinant Rabbit Monoclonal Antibody (107) (Product # MA5-29385, 1:300). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody, counterstained with Alexa Fluor® 546-conjugated phallotoxins (red) and DAPI (blue). Positive staining was localized to lysosome membrane.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Confocal immunofluorescence analysis of Human LAMP1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with LAMP1 Recombinant Rabbit Monoclonal Antibody (107) (Product # MA5-29385, 1:300). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody, counterstained with Alexa Fluor® 546-conjugated phallotoxins (red) and DAPI (blue). Positive staining was localized to lysosome membrane.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of LAMP1 (Lysosome-associated membrane glycoprotein 1) was performed using 80% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with LAMP1 Recombinant Rabbit Monoclonal Antibody (107) (Product # MA5-29385) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2500 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing endosome and lysosome-like staining for LAMP1. Panel e represents control Hep G2 cells with no primary antibody to assess background. The images were captured at 60X magnification.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of Human LAMP1 on Jurkat cells. Cells were treated according to manufacturer’s manual, stained with LAMP1 Recombinant Rabbit Monoclonal Antibody (107) (Product # MA5-29385), then a FITC-conjugated Secondary antibody. The histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

LAMP1 Immunoprecipitation using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 1 µL with LAMP1 Recombinant Rabbit Monoclonal Antibody (107) (Product # MA5-29385) and 15 µL of 50 % Protein G agarose. Primary antibody: LAMP1 Recombinant Rabbit Monoclonal Antibody (107), at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 45 kDa. Observed band size: 113 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fluorescent microscopic images showing colocalization of lysosomal markers LAMP1 and BODIPY 1 in Ehrlich ascitic carcinoma cells: ( A )--BODIPY 1 (red) and nuclei (blue), ( B )--LAMP1 (green) and nuclei (blue), ( C )--overlapping BODIPY 1 and LAMP1 fluorescent staining. Magnification 40x.