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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- GTX19294 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX19294, RRID:AB_423508
- Product name
- LAMP1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references BAX channel activity mediates lysosomal disruption linked to Parkinson disease.
Pathogenic lysosomal depletion in Parkinson's disease.
Bové J, Martínez-Vicente M, Dehay B, Perier C, Recasens A, Bombrun A, Antonsson B, Vila M
Autophagy 2014 May;10(5):889-900
Autophagy 2014 May;10(5):889-900
Pathogenic lysosomal depletion in Parkinson's disease.
Dehay B, Bové J, Rodríguez-Muela N, Perier C, Recasens A, Boya P, Vila M
The Journal of neuroscience : the official journal of the Society for Neuroscience 2010 Sep 15;30(37):12535-44
The Journal of neuroscience : the official journal of the Society for Neuroscience 2010 Sep 15;30(37):12535-44
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of LAMP1 was performed by loading 20 £gg of HeLa cell lysate and 5 £gl of Molecular Weight Protein Ladder per well onto a 4-12% Bis-Tris polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an anti-LAMP1 polyclonal antibody (GTX19294) at a dilution of 1:500 for one hour at room temperature, washed in TBS-0.1%Tween-20, and probed with a goat anti-rabbit IgG-HRP secondary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence staining of LAMP1 (red) in HeLa cells.
