Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- AM03025PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM03025PU-N, RRID:AB_10974381
- Product name
- anti CD107a / LAMP1
- Antibody type
- Monoclonal
- Antigen
- U937 cell line
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- B-T47
- Vial size
- 2ml (200T)
- Concentration
- 0.1 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western Blot analysis using CD107a / LAMP1 Antibody Cat.-No AM03025PU-N (Clone B-T47) at 2 µg/ml in 1 mg/ml lysate, HeLa 10 µg (Lane 1+2), Raw 20µg (Lane 3+4) lysate, blocking buffer (Blotto; 5% dry milk powder in TBST). R1253HRP anti-Mouse IgG (H+L)-HRP as secondary antibody.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- CD107a / LAMP1 Antibody Cat.-No AM03025PU-N (Clone B-T47) on Hela cells at 5 µg/ml (Left), R1404F with F(ab)2 anti Mouse IgG (H+L)-FITC conjugated secondary antibody (1/100). Cells were fixed in 1% PFA, permeabilized in 0.25%Triton X 100 in PBS, blocked in 1% BSA in PBS.Â