IC4800A

antibody from R&D Systems

Targeting: LAMP1 CD107a

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC4800A

Provider

R&D Systems

Product name

Human LAMP-1/CD107a APC-conjugated Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human LAMP-1/CD107a in direct ELISAs and Western blots.

Reactivity

Human

Host

Mouse

Conjugate

Red dye

Antigen sequence

P11279


Isotype

IgG

Antibody clone number

508921

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of LAMP-1/CD107a in THP-1 Human Cell Line by Flow Cytometry. THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human LAMP-1/CD107a APC-conjugated Monoclonal Antibody (Catalog # IC4800A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of LAMP-1/CD107a in Human NK cells by Flow Cytometry. Human NK Cells either (A) stimulated in a 1:1 ratio with K562 human chronic myelogenous leukemia cell line and 3 μM monensin for 5 hours or (B) unstimulated were stained with Mouse Anti-Human LAMP-1/CD107a APC-conjugated Monoclonal Antibody (Catalog # IC4800A) and Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P). Quadrant markers were set based on control antibody staining (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.