PA5-22010
antibody from Invitrogen Antibodies
Targeting: SNU13
15.5K, FA-1, NHP2L1, SNRNP15-5, SPAG12, SSFA1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-22010 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NHP2L1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: 293T, H1299, HeLa, HepG2, Molt-4, Raji. Predicted reactivity: Xenopus laevis (92%), Pig (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NHP2L1 Polyclonal Antibody (Product # PA5-22010). Sample (30 µg of whole cell lysate). A: HeLa. B: Hep G2. 12% SDS PAGE. NHP2L1 Polyclonal Antibody (Product # PA5-22010) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NHP2L1 antibody detects NHP2L1 protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract. B. 30 µg GL261 whole cell lysate/extract. C. 30 µg C8D30 whole cell lysate/extract. D. 30 µg NIH-3T3 whole cell lysate/extract. E. 30 µg BCL-1 whole cell lysate/extract. F. 30 µg Raw264.7 whole cell lysate/extract. G. 30 µg C2C12 whole cell lysate/extract.15 % SDS-PAGE. NHP2L1 antibody NHP2L1 Polyclonal Antibody (Product # PA5-22010) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NHP2L1 antibody detects NHP2L1 protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract. B. 30 µg Rat2 whole cell lysate/extract.15 % SDS-PAGE. NHP2L1 antibody NHP2L1 Polyclonal Antibody (Product # PA5-22010) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NHP2-like protein 1 in paraformaldehyde-fixed HeLa cells using a NHP2-like protein 1 polyclonal antibody (Product # PA5-22010) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NHP2-like protein 1 antibody (Product # PA5-22010) at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NHP2-like protein 1 (Product # PA5-22010) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.