Antibody data
- Antibody Data
- Antigen structure
- References [21]
- Comments [0]
- Validations
- Flow cytometry [2]
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- Product number
- MAB1599-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human MICB Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human MICB in direct ELISAs and Western blots. Does not cross-react with recombinant human MICA.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
CAI18747
- Isotype
- IgG
- Antibody clone number
- 236511
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Purine nucleotide metabolism regulates expression of the human immune ligand MICA.
Increasing TIMP3 expression by hypomethylating agents diminishes soluble MICA, MICB and ULBP2 shedding in acute myeloid leukemia, facilitating NK cell-mediated immune recognition.
MicroRNA-519a-3p mediates apoptosis resistance in breast cancer cells and their escape from recognition by natural killer cells.
Human cytomegalovirus escapes immune recognition by NK cells through the downregulation of B7-H6 by the viral genes US18 and US20.
Combination treatment with decitabine and ionizing radiation enhances tumor cells susceptibility of T cells.
NKG2D-dependent activation of dendritic epidermal T cells in contact hypersensitivity.
Genotoxic Stress Induces Senescence-Associated ADAM10-Dependent Release of NKG2D MIC Ligands in Multiple Myeloma Cells.
Two novel human cytomegalovirus NK cell evasion functions target MICA for lysosomal degradation.
RNA-binding proteins regulate the expression of the immune activating ligand MICB.
MicroRNA editing facilitates immune elimination of HCMV infected cells.
Altered microRNA expression after infection with human cytomegalovirus leads to TIMP3 downregulation and increased shedding of metalloprotease substrates, including MICA.
Cytotoxicity of CD56(bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D, LFA-1 and TRAIL and dampened via CD94/NKG2A.
MiR-10b downregulates the stress-induced cell surface molecule MICB, a critical ligand for cancer cell recognition by natural killer cells.
DNAM-1 ligand expression on Ag-stimulated T lymphocytes is mediated by ROS-dependent activation of DNA-damage response: relevance for NK-T cell interaction.
Rapid expansion and long-term persistence of elevated NK cell numbers in humans infected with hantavirus.
Thermal- and oxidative stress causes enhanced release of NKG2D ligand-bearing immunosuppressive exosomes in leukemia/lymphoma T and B cells.
Inhibition of NKG2D expression in NK cells by cytokines secreted in response to human cytomegalovirus infection.
Obstructing shedding of the immunostimulatory MHC class I chain-related gene B prevents tumor formation.
Cutting edge: the metalloproteinase ADAM17/TNF-alpha-converting enzyme regulates proteolytic shedding of the MHC class I-related chain B protein.
NK cells recognize and lyse Ewing sarcoma cells through NKG2D and DNAM-1 receptor dependent pathways.
Cancer cells become susceptible to natural killer cell killing after exposure to histone deacetylase inhibitors due to glycogen synthase kinase-3-dependent expression of MHC class I-related chain A and B.
McCarthy MT, Moncayo G, Hiron TK, Jakobsen NA, Valli A, Soga T, Adam J, O'Callaghan CA
The Journal of biological chemistry 2018 Mar 16;293(11):3913-3924
The Journal of biological chemistry 2018 Mar 16;293(11):3913-3924
Increasing TIMP3 expression by hypomethylating agents diminishes soluble MICA, MICB and ULBP2 shedding in acute myeloid leukemia, facilitating NK cell-mediated immune recognition.
Raneros AB, Minguela A, Rodriguez RM, Colado E, Bernal T, Anguita E, Mogorron AV, Gil AC, Vidal-Castiñeira JR, Márquez-Kisinousky L, Bulnes PD, Marin AM, Garay MCG, Suarez-Alvarez B, Lopez-Larrea C
Oncotarget 2017 May 9;8(19):31959-31976
Oncotarget 2017 May 9;8(19):31959-31976
MicroRNA-519a-3p mediates apoptosis resistance in breast cancer cells and their escape from recognition by natural killer cells.
Breunig C, Pahl J, Küblbeck M, Miller M, Antonelli D, Erdem N, Wirth C, Will R, Bott A, Cerwenka A, Wiemann S
Cell death & disease 2017 Aug 3;8(8):e2973
Cell death & disease 2017 Aug 3;8(8):e2973
Human cytomegalovirus escapes immune recognition by NK cells through the downregulation of B7-H6 by the viral genes US18 and US20.
Charpak-Amikam Y, Kubsch T, Seidel E, Oiknine-Djian E, Cavaletto N, Yamin R, Schmiedel D, Wolf D, Gribaudo G, Messerle M, Cicin-Sain L, Mandelboim O
Scientific reports 2017 Aug 17;7(1):8661
Scientific reports 2017 Aug 17;7(1):8661
Combination treatment with decitabine and ionizing radiation enhances tumor cells susceptibility of T cells.
Son CH, Lee HR, Koh EK, Shin DY, Bae JH, Yang K, Park YS
Scientific reports 2016 Sep 27;6:32470
Scientific reports 2016 Sep 27;6:32470
NKG2D-dependent activation of dendritic epidermal T cells in contact hypersensitivity.
Nielsen MM, Dyring-Andersen B, Schmidt JD, Witherden D, Lovato P, Woetmann A, Ødum N, Poulsen SS, Havran WL, Geisler C, Bonefeld CM
The Journal of investigative dermatology 2015 May;135(5):1311-1319
The Journal of investigative dermatology 2015 May;135(5):1311-1319
Genotoxic Stress Induces Senescence-Associated ADAM10-Dependent Release of NKG2D MIC Ligands in Multiple Myeloma Cells.
Zingoni A, Cecere F, Vulpis E, Fionda C, Molfetta R, Soriani A, Petrucci MT, Ricciardi MR, Fuerst D, Amendola MG, Mytilineos J, Cerboni C, Paolini R, Cippitelli M, Santoni A
Journal of immunology (Baltimore, Md. : 1950) 2015 Jul 15;195(2):736-48
Journal of immunology (Baltimore, Md. : 1950) 2015 Jul 15;195(2):736-48
Two novel human cytomegalovirus NK cell evasion functions target MICA for lysosomal degradation.
Fielding CA, Aicheler R, Stanton RJ, Wang EC, Han S, Seirafian S, Davies J, McSharry BP, Weekes MP, Antrobus PR, Prod'homme V, Blanchet FP, Sugrue D, Cuff S, Roberts D, Davison AJ, Lehner PJ, Wilkinson GW, Tomasec P
PLoS pathogens 2014 May;10(5):e1004058
PLoS pathogens 2014 May;10(5):e1004058
RNA-binding proteins regulate the expression of the immune activating ligand MICB.
Nachmani D, Gutschner T, Reches A, Diederichs S, Mandelboim O
Nature communications 2014 Jun 13;5:4186
Nature communications 2014 Jun 13;5:4186
MicroRNA editing facilitates immune elimination of HCMV infected cells.
Nachmani D, Zimmermann A, Oiknine Djian E, Weisblum Y, Livneh Y, Khanh Le VT, Galun E, Horejsi V, Isakov O, Shomron N, Wolf DG, Hengel H, Mandelboim O
PLoS pathogens 2014 Feb;10(2):e1003963
PLoS pathogens 2014 Feb;10(2):e1003963
Altered microRNA expression after infection with human cytomegalovirus leads to TIMP3 downregulation and increased shedding of metalloprotease substrates, including MICA.
Esteso G, Luzón E, Sarmiento E, Gómez-Caro R, Steinle A, Murphy G, Carbone J, Valés-Gómez M, Reyburn HT
Journal of immunology (Baltimore, Md. : 1950) 2014 Aug 1;193(3):1344-52
Journal of immunology (Baltimore, Md. : 1950) 2014 Aug 1;193(3):1344-52
Cytotoxicity of CD56(bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D, LFA-1 and TRAIL and dampened via CD94/NKG2A.
Nielsen N, Ødum N, Ursø B, Lanier LL, Spee P
PloS one 2012;7(2):e31959
PloS one 2012;7(2):e31959
MiR-10b downregulates the stress-induced cell surface molecule MICB, a critical ligand for cancer cell recognition by natural killer cells.
Tsukerman P, Stern-Ginossar N, Gur C, Glasner A, Nachmani D, Bauman Y, Yamin R, Vitenshtein A, Stanietsky N, Bar-Mag T, Lankry D, Mandelboim O
Cancer research 2012 Nov 1;72(21):5463-72
Cancer research 2012 Nov 1;72(21):5463-72
DNAM-1 ligand expression on Ag-stimulated T lymphocytes is mediated by ROS-dependent activation of DNA-damage response: relevance for NK-T cell interaction.
Ardolino M, Zingoni A, Cerboni C, Cecere F, Soriani A, Iannitto ML, Santoni A
Blood 2011 May 5;117(18):4778-86
Blood 2011 May 5;117(18):4778-86
Rapid expansion and long-term persistence of elevated NK cell numbers in humans infected with hantavirus.
Björkström NK, Lindgren T, Stoltz M, Fauriat C, Braun M, Evander M, Michaëlsson J, Malmberg KJ, Klingström J, Ahlm C, Ljunggren HG
The Journal of experimental medicine 2011 Jan 17;208(1):13-21
The Journal of experimental medicine 2011 Jan 17;208(1):13-21
Thermal- and oxidative stress causes enhanced release of NKG2D ligand-bearing immunosuppressive exosomes in leukemia/lymphoma T and B cells.
Hedlund M, Nagaeva O, Kargl D, Baranov V, Mincheva-Nilsson L
PloS one 2011 Feb 25;6(2):e16899
PloS one 2011 Feb 25;6(2):e16899
Inhibition of NKG2D expression in NK cells by cytokines secreted in response to human cytomegalovirus infection.
Muntasell A, Magri G, Pende D, Angulo A, López-Botet M
Blood 2010 Jun 24;115(25):5170-9
Blood 2010 Jun 24;115(25):5170-9
Obstructing shedding of the immunostimulatory MHC class I chain-related gene B prevents tumor formation.
Wu JD, Atteridge CL, Wang X, Seya T, Plymate SR
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Jan 15;15(2):632-40
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Jan 15;15(2):632-40
Cutting edge: the metalloproteinase ADAM17/TNF-alpha-converting enzyme regulates proteolytic shedding of the MHC class I-related chain B protein.
Boutet P, Agüera-González S, Atkinson S, Pennington CJ, Edwards DR, Murphy G, Reyburn HT, Valés-Gómez M
Journal of immunology (Baltimore, Md. : 1950) 2009 Jan 1;182(1):49-53
Journal of immunology (Baltimore, Md. : 1950) 2009 Jan 1;182(1):49-53
NK cells recognize and lyse Ewing sarcoma cells through NKG2D and DNAM-1 receptor dependent pathways.
Verhoeven DH, de Hooge AS, Mooiman EC, Santos SJ, ten Dam MM, Gelderblom H, Melief CJ, Hogendoorn PC, Egeler RM, van Tol MJ, Schilham MW, Lankester AC
Molecular immunology 2008 Sep;45(15):3917-25
Molecular immunology 2008 Sep;45(15):3917-25
Cancer cells become susceptible to natural killer cell killing after exposure to histone deacetylase inhibitors due to glycogen synthase kinase-3-dependent expression of MHC class I-related chain A and B.
Skov S, Pedersen MT, Andresen L, Straten PT, Woetmann A, Odum N
Cancer research 2005 Dec 1;65(23):11136-45
Cancer research 2005 Dec 1;65(23):11136-45
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of MICB in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human MICB Monoclonal Antibody (Catalog # MAB1599, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- MICB Specificity is Shown by Flow Cytometry in Knockout Cell Line. MICB knockout K562 human myelogenous leukemia cell line was stained with Mouse Anti-Human MICB Monoclonal Antibody (Catalog # MAB1599, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). No staining in the MICB knockout K562 cell line was observed. View our protocol for Staining Membrane-associated Proteins.