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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Flow cytometry [1]
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- Product number
- R32254 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- MICB Antibody / MHC class I polypeptide-related sequence B
- Antibody type
- Polyclonal
- Description
- This highly specific MICB antibody is suitable for use in Western blot/Flow cytometry applications with human and mouse samples.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the MICB antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat testis, 2) human SW620, 3) A549 and 4) 22RV1 lysate with MICB antibody. Expected/observed molecular weight ~43 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human Jurkat, 2) human K562, 3) human HEL, 4) human A431, 5) human 293T, 6) human SiHa and 7) mouse HEPA1/6 cell lysate with MICB antibody. Predicted molecular weight ~43 kDa but may be observed at higher molecular weights due to glycosylation.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human K562 cells with MICB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MICB antibody.
