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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 15800-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LRCH3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: YQENRGSLV VTNGGVEHDL DQIDYIDSCT AEEEEAEVRQ PKGPDPDSLS SQFMAYIEQR RISHEGSPVK PVAIREFQKT EDMRRYLHQN RVPAEPSSLL SLSASHNQLS HTDLELHQRR EQLVERTRRE AQLAALQYEE EKIRTKQIQR DAVLDFVKQK ASQSPQKQHP LLDGVDGECP FPSRRSQHTD DSALCMSLSG LNQVGCAATL PHSSAFTPLK SDDRPNALLS SPATETVHHS PAYSFPAAIQ RNQPQRPESF LFRAGVRAET NKGHASPLPP SAAPTTDSTD SITGQNSRQR EEELELIDQL RKHIEYRLKV SLPCDLGAAL TDGVVLCHLA NHVRPRSVPS IHVPSPAVVS (354-712 aa encoded by BC007504)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.37 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 15800-1-AP (LRCH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human liver tissue were subjected to SDS PAGE followed by western blot with 15800-1-AP (LRCH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human spleen tissue were subjected to SDS PAGE followed by western blot with 15800-1-AP (LRCH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse lung tissue were subjected to SDS PAGE followed by western blot with 15800-1-AP (LRCH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of A431 cells using 15800-1-AP (LRCH3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-LRCH3 (IP:15800-1-AP, 3ug; Detection:15800-1-AP 1:1000) with mouse liver tissue lysate 12500ug.
