PA1-10022

antibody from Invitrogen Antibodies

Targeting: CORO1A coronin-1, HCORO1, p57

Western blot

Antibody data

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Validation data

Product number: PA1-10022 - Provider product page
Provider: Invitrogen Antibodies
Product name: Coronin 1A Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: PA1-10022 was made against the C-terminal peptide of human coronin 1a chemically coupled to keyhole limpet hemocyanin carrier protein. PA1-10022 is also known to work on many mammalian species both on western blots and on cells grown in tissue culture.
Reactivity: Human, Mouse, Rat, Bovine, Porcine
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: Conc. Not Determined
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

CORO1A protein structure - PA1-10022 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Coronin 1A was performed using 70% confluent log phase RAW 264.7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Coronin 1A Polyclonal Antibody (Product # PA1-10022) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing cytoskeletal localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Coronin 1A using a polyclonal antibody (Product # PA1-10022).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Coronin 1A using a polyclonal antibody (Product # PA1-10022).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with rabbit pAb to coronin 1a (Product # PA1-10022, diluted 1:1,000) in red, and costained with mouse mAb to GFAP (diluted 1:1,000) in green. The blue is Hoechst staining of nuclear DNA. The coronin 1a antibody labels protein expressed in the cytoplasm of microglia cells, while GFAP antibody stains intermediate filaments in astrocytic cells.