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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
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- Product number
- MA5-11188 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SUZ12 Monoclonal Antibody (K.6.9)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- K.6.9
- Vial size
- 100 µL
- Concentration
- 84 µg/mL
- Storage
- -20°C
Submitted references Effects of Pazopanib, Sunitinib, and Sorafenib, Anti-VEGF Agents, on the Growth of Experimental Endometriosis in Rats.
Yildiz C, Kacan T, Akkar OB, Karakus S, Kacan SB, Ozer H, Cetin A
Reproductive sciences (Thousand Oaks, Calif.) 2015 Nov;22(11):1445-51
Reproductive sciences (Thousand Oaks, Calif.) 2015 Nov;22(11):1445-51
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of SUZ12 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with SUZ12 Monoclonal Antibody (K.6.9) (Product # MA5-11188) at 1:800 dilution in 0.1% BSA, incubated at 4-degree Celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess the background. The images were captured at 60X magnification.
