PA5-27427

antibody from Invitrogen Antibodies

Targeting: DDX39B BAT1, D6S81E, UAP56

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27427

Provider

Invitrogen Antibodies

Product name

UAP56 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, HeLa, HepG2.

Concentration

0.43 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

BAT1 antibody detects BAT1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with BAT1 antibody UAP56 Polyclonal Antibody (Product # PA5-27427) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of UAP56 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: UAP56 Polyclonal Antibody (Product # PA5-27427) diluted at 1:500. Red: alpha Tubulin protein stained by alpha Tubulin antibody. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of UAP56 was performed in paraffin-embedded mouse brain tissue using UAP56 Polyclonal Antibody (Product # PA5-27427) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of UAP56 was performed in paraffin-embedded mouse ovary tissue using UAP56 Polyclonal Antibody (Product # PA5-27427) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of UAP56 was performed in paraffin-embedded rat liver tissue using UAP56 Polyclonal Antibody (Product # PA5-27427) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded A549 xenograft , using UAP56 (Product # PA5-27427) antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.