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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PA5-77790 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ULK3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ULK3 in human A549 cell lysates with 15 µg of sample. The sample was incubated with ULK3 polyclonal antibody (Product # PA5-77790) using a dilution of 1:1000 (16 hours at 4°C), and followed by Goat Anti-Rabbit HRP (1 hour at RT) at a dilution of 1:2000 and ECL development (6 min at RT). Samples were arranged as follows: Lane 1: Molecular Weight Ladder (MW), Lane 2: Human A549 cell lysates.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ULK3 in rat brain cell lysates with 15 µg of sample. The sample was blocked with 5% skim milk in TBST, incubated with ULK3 polyclonal antibody (Product # PA5-77790) using a dilution of 1:1000 (1 hour at RT), followed by Goat Anti-Rabbit HRP (1 hour at RT) at a dilution of 1:2000 and ECL development (6 min at RT). Samples were arranged as follows: Lane 1) Molecular Weight Ladder (MW), Lane 2) Rat Brain cell lysates.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ULK3 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with ULK3 polyclonal antibody (Product # PA5-77790) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:200, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain B) Phalloidin Texas Red F-Actin stain C) ULK3 Antibody D) Merged image. Magnification: 60x.
