500-P256BT-1MG

antibody from Invitrogen Antibodies

Targeting: SERPINA12 OL-64, Vaspin

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

500-P256BT-1MG

Provider

Invitrogen Antibodies

Product name

Serpina12 Polyclonal Antibody, Biotin, PeproTech®

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

AA Sequence of recombinant protein: MLKPSFSPRN YKALSEVQGW KQRMAAKELA RQNMDLGFKL LKKLAFYNPG RNIFLSPLSI STAFSMLCLG AQDSTLDEIK QGFNFRKMPE KDLHEGFHYI IHELTQKTQD LKLSIGNTLF IDQRLQPQRK FLEDAKNFYS AETILTNFQN LEMAQKQIND FISQKTHGKI NNLIENIDPG TVMLLANYIF FRARWKHEFD PNVTKEEDFF LEKNSSVKVP MMFRSGIYQV GYDDKLSCTI LEIPYQKNIT AIFILPDEGK LKHLEKGLQV DTFSRWKTLL SRRVVDVSVP RLHMTGTFDL KKTLSYIGVS KIFEEHGDLT KIAPHRSLKV GEAVHKAELK MDERGTEGAA GTGAQTLPME TPLVVKIDKP YLLLIYSEKI PSVLFLGKIV NPIGK. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Vaspin. Anti-Human Vaspin-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hVaspin by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Vaspin (500-P256) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hVaspin. Western Blot: To detect hVaspin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hVaspin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P256BT-1MG will be provided as 2 x 500 µg

Reactivity

Human

Host

Rabbit

Conjugate

Biotin

Vial size

2 x 500 µg

Concentration

0.1-1.0 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot: To detect hVaspin by Western Blot analysis Serpina12 Polyclonal Antibody, Biotin (Product # 500-P256BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hVaspin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Conjugate

Biotin

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot: To detect hVaspin by Western Blot analysis Serpina12 Polyclonal Antibody, Biotin (Product # 500-P256BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hVaspin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Conjugate

Biotin

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sandwich ELISA: To detect hVaspin by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of Serpina12 Polyclonal Antibody, Biotin (Product # 500-P256BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Serpina12 Polyclonal Antibody (Product # 500-P256-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hVaspin.

Conjugate

Biotin