17-3007-42

antibody from Invitrogen Antibodies
Targeting: CD300E CD300LE, CLM2, IREM2
Provider product page for 17-3007-42

Antibody data

Product number
17-3007-42 - Provider product page
Provider
Invitrogen Antibodies
Product name
CD300e (IREM-2) Monoclonal Antibody (UP-H2), APC, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The UP-H2 monoclonal antibody recognizes human IREM-2 (immune receptor expressed in monocytic derived cells) also known as LMIR6, and unofficially CD300E and CD300LE. It is a member of the CMRF-35 family. This 34 kDa Ig-like containing receptor is an activating receptor and through it's charged residues in the cytokplasmic domain interacts with ITAM containing adaptor proteins like DAP12. Expression is found exclusively on monocytes and myeloid DC's. Expression may be transient as differentiation of monocytes leads to down regulation of IREM-2 yet expression is found on mature DCs. It has been also noted that myeloid cell lines such as THP-1 and U937 do not express IREM-2. In vitro addition of UP-H2 antibody either soluble or crosslinked stimulates monocytes. Applications Reported: This UP-H2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This UP-H2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
UP-H2
Vial size
100 Tests
Concentration
5 µL/Test
Storage
4° C, store in dark, DO NOT FREEZE!
CD300E protein structure - 17-3007-42 shown in red.