Explore
Validate
Learn
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 17-3007-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD300e (IREM-2) Monoclonal Antibody (UP-H2), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The UP-H2 monoclonal antibody recognizes human IREM-2 (immune receptor expressed in monocytic derived cells) also known as LMIR6, and unofficially CD300E and CD300LE. It is a member of the CMRF-35 family. This 34 kDa Ig-like containing receptor is an activating receptor and through it's charged residues in the cytokplasmic domain interacts with ITAM containing adaptor proteins like DAP12. Expression is found exclusively on monocytes and myeloid DC's. Expression may be transient as differentiation of monocytes leads to down regulation of IREM-2 yet expression is found on mature DCs. It has been also noted that myeloid cell lines such as THP-1 and U937 do not express IREM-2.
- Antibody clone number
- UP-H2
- Concentration
- 5 µL/Test
Submitted references The transition of M-CSF-derived human macrophages to a growth-promoting phenotype.
IREM-1 is a novel inhibitory receptor expressed by myeloid cells.
Molecular characterization of a novel immune receptor restricted to the monocytic lineage.
Hamidzadeh K, Belew AT, El-Sayed NM, Mosser DM
Blood advances 2020 Nov 10;4(21):5460-5472
Blood advances 2020 Nov 10;4(21):5460-5472
IREM-1 is a novel inhibitory receptor expressed by myeloid cells.
Alvarez-Errico D, Aguilar H, Kitzig F, Brckalo T, Sayós J, López-Botet M
European journal of immunology 2004 Dec;34(12):3690-701
European journal of immunology 2004 Dec;34(12):3690-701
Molecular characterization of a novel immune receptor restricted to the monocytic lineage.
Aguilar H, Alvarez-Errico D, García-Montero AC, Orfao A, Sayós J, López-Botet M
Journal of immunology (Baltimore, Md. : 1950) 2004 Dec 1;173(11):6703-11
Journal of immunology (Baltimore, Md. : 1950) 2004 Dec 1;173(11):6703-11
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD85d (ILT4) FITC (Product # 11-5148-42) and Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (left) or Anti-Human CD300e (IREM-2) APC (right). Cells in the monocyte gate were used for analysis.
