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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-53324 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD300e Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: TVWCQYESMY KGYNKYWCRG QYDTSCESIV ETKGEEKVER NGRVSIRDHP EALAFTVTMQ NLNEDDAGSY WCKIQTVWVL DSWSRDPSDL VRVYVSPAIT TPRRTTHPAT PPIFLVVNPG RNLSTGEVLT QNSGFR Highest antigen sequence identity to the following orthologs: Mouse - 55%, Rat - 56%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD300e in control (vector only transfected HEK293T lysate) and CD300E over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a CD300e Polyclonal Antibody (Product # PA5-53324).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CD300e in human lung using CD300e Polyclonal Antibody (Product # PA5-53324) shows moderate to strong cytoplasmic positivity in macrophages.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CD300e in human skeletal muscle using CD300e Polyclonal Antibody (Product # PA5-53324) shows no positivity in myocytes as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CD300e in human bone marrow using CD300e Polyclonal Antibody (Product # PA5-53324) shows moderate cytoplasmic positivity in subsets of hematopoietic cells.
