HPA015570

antibody from Atlas Antibodies

Targeting: CD300E CD300LE, CLM2, IREM2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA015570

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA015570, RRID:AB_1846294

Product name

Anti-CD300E

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

TVWCQYESMYKGYNKYWCRGQYDTSCESIVETKGE
EKVERNGRVSIRDHPEALAFTVTMQNLNEDDAGSY
WCKIQTVWVLDSWSRDPSDLVRVYVSPAITTPRRT
THPATPPIFLVVNPGRNLSTGEVLTQNSGFR


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and CD300E over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY405761).

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human bone marrow shows moderate cytoplasmic positivity in subset of hematopoietic cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human lung shows moderate to strong cytoplasmic positivity in macrophages.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows no positivity in myocytes as expected.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human bone marrow shows moderate cytoplasmic positivity in subsets of hematopoietic cells.

Sample type

HUMAN