PA5-56565

antibody from Invitrogen Antibodies

Targeting: YKT6

Western blot (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-56565

Provider

Invitrogen Antibodies

Product name

YKT6 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: LCHVYVRNDS LAGVVIADNE YPSRVAFTLL EKVLDEFSKQ VDRIDWPVGS PATIHYPALD GHLSRYQ Highest antigen sequence identity to the following orthologs: Mouse - 90%, Rat - 96%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Tumor Suppressor miR-584-5p Inhibits Migration and Invasion in Smoking Related Non-Small Cell Lung Cancer Cells by Targeting YKT6.

Lee SB, Park YS, Sung JS, Lee JW, Kim B, Kim YH
Cancers 2021 Mar 8;13(5)

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 7 YKT6 is a direct target gene of miR-584-5p in smoking-related NSCLC cells. ( A ) miR DB (target score >= 80) and Targetscan database were used to analyze the target gene of miR-584-5p. Venn diagrams show groups of predicted target genes. ( B ) Verification of the mRNA expression of candidate target genes by qRT-PCR in H1703 and A549 cells transfected with miR-584-5p mimic or miR control; * p < 0.05, ** p < 0.01, and *** p < 0.001. ( C ) Verification of the protein expression of candidate target genes (HDAC1, YKT6, RAP2A, and ENAH) by Western blot assays in H1703 and A549 cells transfected with the miR-584-5p mimic or miR control; * p < 0.05 and ** p < 0.01. Full Western Blot images can be found in Figure S2 . ( D ) Representative images of the has-miR-584/YKT6 binding sequence. ( E ) Relative luciferase activity was measured in H1703 and A549 cells transfected with the reporter vector containing wild-type or mutant-type YKT6 3'UTR (untranslated region), along with miR-584-5p mimic or miR control; ** p < 0.01. ( F , G ) Expression of YKT6 in xenograft tumor tissue was analyzed by Western blot and immunohistochemical (IHC) staining. Full Western Blot images can be found in Figure S3 .