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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-60110 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NDUFB9 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: FWYRQHPQPY IFPDSPGGTS YERYDCYKVP EWCLDDWHPS EKAMYPDYFA KREQWKKLRR ESWEREVKQL Q
- Concentration
- 0.05 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NDUFB9 in Lane 1: Mouse liver tissue. lysate; Lane 2: Rat liver tissue. lysate. Samples were probed using a NDUFB9 Polyclonal Antibody (Product # PA5-60110).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NDUFB9 in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted); Lane 5: Human liver tissue. Samples were probed using a NDUFB9 Polyclonal Antibody (Product # PA5-60110).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot was performed using Anti-NDUFB9 Polyclonal Antibody (Product # PA5-60110) and a 22 kDa band corresponding to NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 was observed. Whole cell extracts (30 µg lysate) of SK-BR-3 (Lane 1), MCF7 (Lane 2), MDA-MB-231 (Lane 3), Hep G2 (Lane 4), Jurkat (Lane 5), A549 (Lane 6), COS-7 (Lane 7) and tissue extracts (30 µg lysate) of Mouse Spleen (Lane 8), Rat Spleen (Lane 9), Mouse Heart (Lane 10), Rat Heart (Lane 11) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.2 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Relative expression was observed between SK-BR-3, MCF7 and MDA-MB-231 as expected (https://doi.org/10.1371/journal.pone.0144441).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of NDUFB9 in human cell line A-431 shows positivity in mitochondria. Samples were probed using a NDUFB9 Polyclonal Antibody (Product # PA5-60110).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NDUFB9 in human kidney tissue shows strong cytoplasmic positivity in cells in tubules. Samples were probed using a NDUFB9 Polyclonal Antibody (Product # PA5-60110).
