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Western blot
ELISA
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- PA5-143971 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD89 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL. Positive Control - WB: human HL-60 whole cell, human U-937 whole cell, human Jurkat whole cell. ICC/IF: Hela cell. Flow: Hela cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 500 µg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of CD89 in Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in CD89 Polyclonal antibody (Product # PA5-143971) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of CD89 in HeLa cells (Blue line). The cells were blocked with 10% normal goat serum, and then incubated with CD89 polyclonal antibody (Product # PA5-143971) (1 µg/1x10^6 cells) for 30 min at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10^6 cells) used under the same conditions. Unlabeled sample (Red line) was also used as a control. DyLight®488 conjugated goat anti-rabbit IgG (5-10 μg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C.
