Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NBP2-29409 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Cytokeratin 18 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Hamster, Porcine, Sheep
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Analysis of Cytokeratin 18 in HeLa lysate using Cytokeratin 18 (CK18) antibody at 1 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Simple Western lane view shows a specific band for Cytokeratin 18 in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 18 antibody was used at 10 ug/ml dilution of HeLa lysates(s) respectively.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Western Blot Analysis of HCT116 cell lysate using Cytokeratin 18 Antibody (DA7).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Formalin-fixed, paraffin-embedded human colon cancer stained with anti-Cytokeratin 18 (CK18) at 1:200, using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of tumor cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Formalin-paraffin human colon cancer stained with Cytokeratin 18 Ab (DA7). Note cytoplasmic staining of tumor cells.