PAB28459

antibody from Abnova Corporation

Targeting: KRT18

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28459

Provider

Abnova Corporation

Product name

KRT18 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant KRT18.

Antigen sequence

IEESTTVVTTQSTEVGAAKAMLTELTRTVQFLEIN
LDSMRNLKASLENSLREVVARYALQMEQLNGILLH
LESELAQTWAEGQCQAQEYQALLNIKVKLEAAIAT
YRRLLEDG


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: EFO-21, Lane 3: A-431, Lane 4: Liver, Lane 5: Tonsil with KRT18 polyclonal antibody (Cat # PAB28459) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of U-2 OS with KRT18 polyclonal antibody (Cat # PAB28459) at 1-4 ug/mL dilution shows positivity in cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human placenta with KRT18 polyclonal antibody (Cat # PAB28459) shows distinct membranous and cytoplasmic positivity in trophoblastic cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)