LS-C285704

antibody from LSBio

Targeting: KRT18

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Antibody Data

Product number

LS-C285704

Provider

LSBio

Product name

KRT18 / CK18 / Cytokeratin 18 Antibody LS-C285704

Antibody type

Polyclonal

Description

Caprylic acid and ammonium sulfate precipitation

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot All lanes: KRT18 antibody at 12µg/ml Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: Zebrafish lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 49 kDa Observed band size: 49 kDa

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot All lanes: KRT18 antibody at 2µg/ml Lane 1: K562 whole cell lysate Lane 2: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 49 kDa Observed band size: 49 kDa

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot All lanes: KRT18 antibody at 2µg/ml Lane 1: K562 whole cell lysate Lane 2: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 49 kDa Observed band size: 49 kDa

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western Blot Positive WB detected in: K562 whole cell lysate, Hela whole cell lysate All lanes: KRT18 antibody at 3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 49 kDa Observed band size: 49 kDa

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot All lanes: KRT18 antibody at 12µg/ml Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: Zebrafish lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 49 kDa Observed band size: 49 kDa