Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP2-33082 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Cytokeratin 18 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Hamster, Porcine, Sheep
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Cytokeratin 18 Antibody (DC10) - Azide and BSA Free [NBP2-33082] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 18 antibody was used at 10 ug/ml dilution of HeLa lysates(s) respectively.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Cytokeratin 18 Antibody (DC10) - Azide and BSA Free [NBP2-33082] - Simple Western lane view shows a specific band for Cytokeratin 18 in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cytokeratin 18 Antibody (DC10) - Azide and BSA Free [NBP2-33082] - Western Blot Analysis of human Intestine tissue lysate using Cytokeratin 18 Antibody (DC10).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cytokeratin 18 Antibody (DC10) - Azide and BSA Free [NBP2-33082] - Western Blot Analysis of human HCT116 cell lysate using Cytokeratin 18 Antibody (DC10).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cytokeratin 18 Antibody (DC10) - Azide and BSA Free [NBP2-33082] - Analysis of Keratin 18 in 1) HeLa and 2) A431 lysate using Keratin 18 antibody at 1 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (DC10) - Azide and BSA Free [NBP2-33082] - Staining of human skin sweat gland. Note cytoplasmic staining of tumor cells.