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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- GTX112978 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX112978, RRID:AB_1950644
- Product name
- Cytokeratin 18 antibody [N3C3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Profiling human breast epithelial cells using single cell RNA sequencing identifies cell diversity.
Nguyen QH, Pervolarakis N, Blake K, Ma D, Davis RT, James N, Phung AT, Willey E, Kumar R, Jabart E, Driver I, Rock J, Goga A, Khan SA, Lawson DA, Werb Z, Kessenbrock K
Nature communications 2018 May 23;9(1):2028
Nature communications 2018 May 23;9(1):2028
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (15 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody [N3C3] (GTX112978) diluted at 1:8000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: Raji 10% SDS PAGE GTX112978 diluted at 1:10000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (15 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody [N3C3] (GTX112978) diluted at 1:8000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 18 (GTX112978) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded H661 xenograft, using Cytokeratin 18(GTX112978) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cytokeratin 18 antibody [N3C3] detects Cytokeratin 18 protein at cytoplasm in mouse urinary bladder by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder. Cytokeratin 18 antibody [N3C3] (GTX112978) diluted at 1:500.
