Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-36798 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-Cytokeratin 18 (Ser52) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 46 kDa. Purity is >95% by SDS-PAGE.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-Cytokeratin 18 (Ser52) in Lane 1: HeLa cell lysate, Lane 2: NIH-3T3 cell lysate, Lane 3: PC12 cell lysate. Samples were incubated with Phospho-Cytokeratin 18 (Ser52) polyclonal antibody (Product # PA5-36798) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-Cytokeratin 18 pSer52 using Phospho-Cytokeratin 18 pSer52 polyclonal antibody (Product # PA5-36798) at a dilution of 1:500. Lane 1: Hela cell lysate, Lane 2: NIH-3T3 cell lysate, Lane 3: PC12 cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Phospho-Cytokeratin 18 (Ser52) in paraffin-embedded human breast carcinoma tissue (cytoplasmic staining) and negative control (right, with PBS only). Samples were incubated with Phospho-Cytokeratin 18 (Ser52) polyclonal antibody (Product # PA5-36798) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.