Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-44947-0.1mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Cytokeratin 18 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified. This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
- Reactivity
- Human, Rat, Canine, Feline
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Cytokeratin 18 Antibody (DE-K18) [NBP2-44947] - Simple Western lane view shows a specific band for Cytokeratin 18 in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Cytokeratin 18 Antibody (DE-K18) [NBP2-44947] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 18 antibody was used at 10 ug/ml dilution of HeLa lysates(s) respectively.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cytokeratin 18 Antibody (DE-K18) [NBP2-44947] - Western Blot Analysis of human HCT116 cell lysate using Cytokeratin 18 Antibody (DE-K18).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cytokeratin 18 Antibody (DE-K18) [NBP2-44947] - Western Blot Analysis of MCF-7cell lysate using Cytokeratin 18 Antibody (DE-K18).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (DE-K18) [NBP2-44947] - Human Colon Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (DE-K18).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Cytokeratin 18 Antibody (DE-K18) [NBP2-44947] - Flow Cytometric Analysis of trypsinised MeOH-fixed HeLa cells using Cytokeratin 18 Antibody (DE-K18) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).