Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- V2178 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Cytokeratin 18 Antibody
- Antibody type
- Monoclonal
- Antigen
- Human breast cancer PMC 42 cells were used as the immunogen for this antibody.
- Description
- Protein G purified antibody
- Reactivity
- Human, Rat, Bovine, Canine, Hamster, Porcine
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- DA7
- Vial size
- 20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)
- Storage
- Store the Cytokeratin 18 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Submitted references Quantitative proteomic profiling of tumor cell response to telomere dysfunction using isotope-coded protein labeling (ICPL) reveals interaction network of candidate senescence markers.
Database on monoclonal antibodies to cytokeratins.
Monoclonal antibodies recognizing different epitopes of cytokeratin No.18.
Biniossek ML, Lechel A, Rudolph KL, Martens UM, Zimmermann S
Journal of proteomics 2013 Oct 8;91:515-35
Journal of proteomics 2013 Oct 8;91:515-35
Database on monoclonal antibodies to cytokeratins.
Upasani OS, Vaidya MM, Bhisey AN
Oral oncology 2004 Mar;40(3):236-56
Oral oncology 2004 Mar;40(3):236-56
Monoclonal antibodies recognizing different epitopes of cytokeratin No.18.
Vojtĕsek B, Stasková Z, Nenutil R, Lauerová L, Kovarík J, Rejthar A, Bártková J, Bártek J
Folia biologica 1989;35(6):373-82
Folia biologica 1989;35(6):373-82
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human samples using Cytokeratin 18 antibody (DA7).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of colon cancer stained with Cytokeratin 18 antibody (DA7). Note cytoplasmic staining of tumor cells.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free Cytokeratin 18 Antibody (clone DA7). Confirmation of Integrity and Purity of the Antibody.