F50352

antibody from NSJ Bioreagents

Targeting: KRT18

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F50352

Provider

NSJ Bioreagents

Product name

Cytokeratin-18 Antibody

Antibody type

Polyclonal

Antigen

A portion of amino acids 401-430 from the human protein was used as the immunogen for this Cytokeratin-18 antibody.

Description

Purified antibody

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Vial size

80 µl, 400 µl

Storage

Aliquot the Cytokeratin-18 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of Cytokeratin-18 antibody and (1) K562, (2) NCI-H460, and (3) mouse stomach lysate.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) NCI-H460, 2) K562, 3) Hela and 4) A431 whole cell lysate with Cytokeratin-18 antibody at 1:2000. Predicted molecular weight ~48 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of Cytokeratin-18 antibody with prostate carcinoma followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of Cytokeratin-18 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC analysis of FFPE human colon carcinoma with Cytokeratin-18 antibody

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Cytokeratin-18 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC analysis of FFPE human liver tissue with Cytokeratin-18 antibody. Required HIER: steamed antigen retrieval with pH6 citrate buffer.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometric analysis of WiDr cells using Cytokeratin-18 antibody (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

FACS testing of fixed and permeabilized human HeLa cells with Cytokeratin-18 antibody (green) at 1:25 and negative control (blue).