Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00003875-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00003875-M01, RRID:AB_539996
- Product name
- Mouse Monoclonal Cytokeratin 18 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. KRT18 - keratin 18
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cytokeratin 18 Antibody (2F8) [H00003875-M01] - Analysis of KRT18 expression in transfected 293T cell line by KRT18 monoclonal antibody (M01), clone 2F8.Lane 1: KRT18 transfected lysate(48.1 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cytokeratin 18 Antibody (2F8) [H00003875-M01] - KRT18 monoclonal antibody (M01), clone 2F8 Analysis of KRT18 expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Cytokeratin 18 Antibody (2F8) [H00003875-M01] - Detection limit for recombinant GST tagged KRT18 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (2F8) [H00003875-M01] - Analysis of monoclonal antibody to KRT18 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Cytokeratin 18 Antibody (2F8) [H00003875-M01] - Analysis of KRT18 transfected lysate using anti-KRT18 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with KRT18 MaxPab rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (2F8) [H00003875-M01] - Analysis of monoclonal antibody to KRT18 on formalin-fixed paraffin-embedded human liver tissue. Antibody concentration 3 ug/ml.