Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunocytochemistry [1]
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- Product number
- 41-9815-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cytokeratin 18 Monoclonal Antibody (LDK18), eFluor™ 570, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody LDK18 recognizes human Cytokeratin 18. Cytokeratin 18 is member of the large family of intermediate filaments found within epithelial cells. The family of cytokeratins is made up of type I (acidic) and type II (basic) members which form heterodimers by pairing an acidic with a basic member to compose the keratin filament. Cytokeratin 18 (type I) is most commonly found associated with cytokeratin 8 (type II). Because many tumors of epithelial origin retain expression of keratin proteins, cytokeratin 18 has been used as a tumor marker to identify both tumors and metastases. In addition, cytokeratin 18 has been shown to be expressed in highly proliferative retinal pigment epithelium cells. Applications Reported: This LDK18 antibody has been reported for use in microscopy, immunohistochemical staining, and immunocytochemistry. Applications Tested: This LDK18 antibody has been tested by immunocytochemistry of MeOH-fixed human cells and can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable. Excitation: 555 nm; Emission: 570 nm
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- LDK18
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of fixed MCF-7 cells stained with 1 µg/mL Mouse IgG1 Isotype Control eFluor® 570 (left) or 1 µg/mL Anti-Human Cytokeratin 18 eFluor® 570 (right). Nuclei are stained with DAPI.