NBP1-47817

antibody from Novus Biologicals

Targeting: KRT18

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-47817

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-47817, RRID:AB_10010788

Product name

Mouse Monoclonal Cytokeratin 18 Antibody

Antibody type

Monoclonal

Description

Affinity purified. This antibody is specific for Homo sapiens keratin 18 (KRT18), transcript variant 2.

Reactivity

Human, Simian

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Concentration

0.6 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Cytokeratin 18 Antibody (1E1) [NBP1-47817] - Cytokeratin 18 antibody ( 1E1) at 1:1000 dilution with HepG2 cell lysate

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Cytokeratin 18 Antibody (1E1) [NBP1-47817] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT18 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT18.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Cytokeratin 18 Antibody (1E1) [NBP1-47817] Analysis of extracts (35ug) from 9 different cell lines by using anti-KRT18 monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Extracts (10ug) from 10 Human tissue by using Cytokeratin 18 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (1E1) [NBP1-47817] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-KRT18 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (1E1) [NBP1-47817] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-KRT18 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (1E1) [NBP1-47817] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-KRT18 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (1E1) [NBP1-47817] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-KRT18 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (1E1) [NBP1-47817] - Staining of paraffin-embedded Human breast tissue using anti-KRT18 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (1E1) [NBP1-47817] - Staining of paraffin-embedded Human colon tissue using anti-KRT18 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (1E1) [NBP1-47817] - Staining of paraffin-embedded Human endometrium tissue using anti-KRT18 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (1E1) [NBP1-47817] - Staining of paraffin-embedded Human Kidney tissue using anti-KRT18 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (1E1) [NBP1-47817] - Staining of paraffin-embedded Human pancreas tissue using anti-KRT18 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Human liver tissue within the normal limits. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3 min, (1:6000).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Human pancreas tissue within the normal limits. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (OTI1E1) [NBP1-47817] - Staining of paraffin-embedded Human colon tissue within the normal limits. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.