MA1-06326

antibody from Invitrogen Antibodies

Targeting: KRT18

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-06326

Provider

Invitrogen Antibodies

Product name

Cytokeratin 18 Monoclonal Antibody (RGE53)

Antibody type

Monoclonal

Antigen

Other

Description

MA1-06326 detects cytokeratin 18 in human, mouse, rat, hamster, rabbit, chicken, canine, zebrafish and swine samples. MA1-06326 has sucessfully been used in Western blotting, immunocytochemistry, flow cytometry, and immunohistochemistry. The MA1-06326 immunogen is a cytoskeletal preparation from cells. Store at 4ºC or in small aliquots at -20ºC.

Reactivity

Human, Mouse, Rat, Canine, Chicken/Avian, Hamster, Porcine, Rabbit, Zebrafish

Host

Mouse

Isotype

IgG

Antibody clone number

RGE53

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Uptake of Manganese from the Manganese-Lysine Complex in Primary Chicken Intestinal Epithelial Cells.

Bai S, Zhang K, Ding X, Wang J, Zeng Q, Peng H, Bai J, Xuan Y, Su Z, Wu B
Animals : an open access journal from MDPI 2019 Aug 15;9(8)

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Polyethylene glycol (PEG)-induced mouse model of choroidal neovascularization.

Lyzogubov VV, Tytarenko RG, Liu J, Bora NS, Bora PS
The Journal of biological chemistry 2011 May 6;286(18):16229-37

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot detection of cytokeratin 18 in cytoskeletal preparations from bovine lens tissue using Product # MA1-06326.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Cytokeratin 18 Monoclonal Antibody (RGE53)(Product # MA1-06326) and a 48kDa band corresponding to Cytokeratin 18 was observed across the cell lines tested except IMR-32 and A-375. Whole cell extracts (30 µg lysate) of A549 (Lane 1), HeLa (Lane 2), Caco-2 (Lane 3), IMR-32 (Lane 4) and A-375 (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunostaining of Cytokeratin 18 in the glandular part of the human cervix using Product # MA1-06326.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Cytokeratin 18 was performed using 70% confluent log phase Caco-2 cells. The cells were fixed with ice-cold acetone at 4°C for 5 minutes and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Cytokeratin 18 Monoclonal Antibody (RGE53) (Product # MA1-06326) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoskeletal localization. Panel e represents A-375 cells which lack cytokeratin 18 expression. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of 5 days old zebrafish embryo. using Cytokeratin 18 monoclonal antibody (Product # MA1-06326). Left panel: DAPI-staining of cell nuclei, providing an overview of the tissue section used for immunostaining

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of 1 month old zebrafish embryo using Cytokeratin 18 monoclonal antibody (Product # MA1-06326).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of epidermis of 2 days old zebrafish embryo using Cytokeratin 18 monoclonal antibody (Product # MA1-06326).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of epidermis of 2 days old zebrafish embryo using Cytokeratin 18 monoclonal antibody (Product # MA1-06326).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of 5 days old zebrafish embryo. using Cytokeratin 18 monoclonal antibody (Product # MA1-06326). Left panel: DAPI-staining of cell nuclei, providing an overview of the tissue section used for immunostaining

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of 1 month old zebrafish embryo using Cytokeratin 18 monoclonal antibody (Product # MA1-06326).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry on frozen section of human colon stained with Cytokeratin 18 monoclonal antibody (Product # MA1-06326).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry on frozen section of human kidney epithelium stained with Cytokeratin 18 monoclonal antibody (Product # MA1-06326).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry on frozen section of swine liver hepatocytes stained with Cytokeratin 18 monoclonal antibody (Product # MA1-06326).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Morphological features and immunofluorescence staining of primary chicken intestinal epithelial cells (IECs). ( A ) Epithelial cells were isolated in cell clusters. ( B ) Primary chicken IECs at 24 h post-seeding. The image shows cobblestone-shaped IECs radially growing from the center of the crypt (Cr) and cell clusters connecting. ( C ) Primary chicken IECs culture within 4-5 days post-seeding. Image shows a confluent chicken IECs. ( D , G , J ) Nuclei were stained (blue). ( E ) Immunostaining against cytokeratin-18 (green) at 3 days post-seeding. ( F ) Immunostaining against vimentin (red, the arrows in the figure) at 3 days post-seeding. ( H ) Primary chicken IECs presented in the image ( G ) were incubated only with the primary antibody of cytokeratin without the secondary antibody to serve as a primary antibody control. ( I ) Primary chicken IECs presented in the image ( G ) were incubated first with buffer only without the primary antibody of cytokeratin-18 and subsequently with secondary antibody to serve as a secondary antibody control. ( K ) Primary chicken IECs presented in the image ( J ) were incubated only with the primary antibody of cytokeratin without the secondary antibody to serve as a primary antibody control. ( L ) Primary chicken IECs presented in the image ( J ) were incubated first with buffer only without the primary antibody of cytokeratin-18 and subsequently with secondary antibody to serve as a secondary antibody control.