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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Flow cytometry [2]
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- Product number
- MA1-10326 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cytokeratin 18 Monoclonal Antibody (C-04), FITC
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This antibody reacts with cytokeratin 18 (45 kDa intracellular antigen), a member of intermediate filaments subfamily represented in epithelial tissues.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- C-04
- Vial size
- 100 µg
- Concentration
- 0.1 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references DHX15 is required to control RNA virus-induced intestinal inflammation.
Xing J, Zhou X, Fang M, Zhang E, Minze LJ, Zhang Z
Cell reports 2021 Jun 22;35(12):109205
Cell reports 2021 Jun 22;35(12):109205
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Cytokeratin 18 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed and permeabilized with ice-cold acetone and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin 18 Monoclonal Antibody (C04), FITC (Product # MA1-10326) at 1:100 dilution in 0.1% BSA and incubated at 4 degree Celsius overnight (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing cytoskeletal localization. Panel e represents the isotype control. Panel f shows the negative cell line A-375 with no Cytokeratin 18 expression. The images were captured at 60X magnification.
- Conjugate
- Green dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Intracellular flow cytometry analysis of cytokeratin expression in HeLa human cervix carcinoma cell line using anti-Cytokeratin 18 (C-04) FITC. Overlay with Isotype mouse IgG1 control (PPV-06)
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis (intracellular staining) of cytokeratin expression in HeLa human cervix carcinoma cell line using anti-Cytokeratin 18 (C-04) FITC (red) Monoclonal antibody (Product # MA1-10326). Overlay with Isotype mouse IgG1 control (PPV-06) FITC antiobody.
- Conjugate
- Green dye
