MA1-39483

antibody from Invitrogen Antibodies

Targeting: KRT18

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-39483

Provider

Invitrogen Antibodies

Product name

Cytokeratin 18 Monoclonal Antibody (DC10)

Antibody type

Monoclonal

Antigen

Other

Description

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is breast carcinoma or skin tissue.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

DC10

Vial size

1 mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Elevated ACE-2 expression in the olfactory neuroepithelium: implications for anosmia and upper respiratory SARS-CoV-2 entry and replication.

Chen M, Shen W, Rowan NR, Kulaga H, Hillel A, Ramanathan M Jr, Lane AP
The European respiratory journal 2020 Sep;56(3)

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Casein kinase 1 is recruited to nuclear speckles by FAM83H and SON.

Kuga T, Kume H, Adachi J, Kawasaki N, Shimizu M, Hoshino I, Matsubara H, Saito Y, Nakayama Y, Tomonaga T
Scientific reports 2016 Sep 29;6:34472

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FAM83H and casein kinase I regulate the organization of the keratin cytoskeleton and formation of desmosomes.

Kuga T, Sasaki M, Mikami T, Miake Y, Adachi J, Shimizu M, Saito Y, Koura M, Takeda Y, Matsuda J, Tomonaga T, Nakayama Y
Scientific reports 2016 May 25;6:26557

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Modelling breast cancer requires identification and correction of a critical cell lineage-dependent transduction bias.

Hines WC, Yaswen P, Bissell MJ
Nature communications 2015 Apr 21;6:6927

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Alterations of canalicular ATP-binding cassette transporter expression in drug-induced liver injury.

Zollner G, Thueringer A, Lackner C, Fickert P, Trauner M
Digestion 2014;90(2):81-8

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A novel mechanism of keratin cytoskeleton organization through casein kinase Iα and FAM83H in colorectal cancer.

Kuga T, Kume H, Kawasaki N, Sato M, Adachi J, Shiromizu T, Hoshino I, Nishimori T, Matsubara H, Tomonaga T
Journal of cell science 2013 Oct 15;126(Pt 20):4721-31

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Glucose and SIRT2 reciprocally mediate the regulation of keratin 8 by lysine acetylation.

Snider NT, Leonard JM, Kwan R, Griggs NW, Rui L, Omary MB
The Journal of cell biology 2013 Feb 4;200(3):241-7

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Malignant transformation of adenomyoepithelioma of the breast by a monophasic population: a report of two cases and review of literature.

Marian C, Boila A, Soanca D, Malau M, Podeanu DM, Resetkova E, Stolnicu S
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2013 Apr;121(4):272-9

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Expression of KRT7 and WT1 differentiates precursor lesions of Wilms' tumours from those of papillary renal cell tumours and mucinous tubular and spindle cell carcinomas.

Szponar A, Kovacs G
Virchows Archiv : an international journal of pathology 2012 Apr;460(4):423-7

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Keratin hypersumoylation alters filament dynamics and is a marker for human liver disease and keratin mutation.

Snider NT, Weerasinghe SV, Iñiguez-Lluhí JA, Herrmann H, Omary MB
The Journal of biological chemistry 2011 Jan 21;286(3):2273-84

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Directed differentiation of human embryonic stem cells to epidermal progenitors.

Metallo CM, Ji L, de Pablo JJ, Palecek SP
Methods in molecular biology (Clifton, N.J.) 2010;585:83-92

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Giant clear cell hidradenoma of the knee.

Yu G, Goodloe S Jr, D'Angelis CA, McGrath BE, Chen F
Journal of cutaneous pathology 2010 Sep;37(9):e37-41

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Cytoskeletal keratin glycosylation protects epithelial tissue from injury.

Ku NO, Toivola DM, Strnad P, Omary MB
Nature cell biology 2010 Sep;12(9):876-85

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Antagonistic roles of Notch and p63 in controlling mammary epithelial cell fates.

Yalcin-Ozuysal O, Fiche M, Guitierrez M, Wagner KU, Raffoul W, Brisken C
Cell death and differentiation 2010 Oct;17(10):1600-12

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Endometrial profile of tamoxifen and low-dose estradiol combination therapy.

Wood CE, Kaplan JR, Fontenot MB, Williams JK, Cline JM
Clinical cancer research : an official journal of the American Association for Cancer Research 2010 Feb 1;16(3):946-56

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Imaging and analysis of 3D tumor spheroids enriched for a cancer stem cell phenotype.

Robertson FM, Ogasawara MA, Ye Z, Chu K, Pickei R, Debeb BG, Woodward WA, Hittelman WN, Cristofanilli M, Barsky SH
Journal of biomolecular screening 2010 Aug;15(7):820-9

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Assessment of the tumorigenesis and drug susceptibility of three new canine mammary tumor cell lines.

Chang CY, Chiou PP, Chen WJ, Li YH, Yiu JC, Cheng YH, Chen SD, Lin CT, Lai YS
Research in veterinary science 2010 Apr;88(2):285-93

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Antigen expression of human eccrine sweat glands.

Li HH, Zhou G, Fu XB, Zhang L
Journal of cutaneous pathology 2009 Mar;36(3):318-24

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Long-term cultures of bone marrow-derived human mesenchymal stem cells frequently undergo spontaneous malignant transformation.

Røsland GV, Svendsen A, Torsvik A, Sobala E, McCormack E, Immervoll H, Mysliwietz J, Tonn JC, Goldbrunner R, Lønning PE, Bjerkvig R, Schichor C
Cancer research 2009 Jul 1;69(13):5331-9

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Frzb, a secreted Wnt antagonist, decreases growth and invasiveness of fibrosarcoma cells associated with inhibition of Met signaling.

Guo Y, Xie J, Rubin E, Tang YX, Lin F, Zi X, Hoang BH
Cancer research 2008 May 1;68(9):3350-60

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Monitoring of epithelial cell caspase activation via detection of durable keratin fragment formation.

Tao GZ, Li DH, Zhou Q, Toivola DM, Strnad P, Sandesara N, Cheung RC, Hong A, Omary MB
The Journal of pathology 2008 Jun;215(2):164-74

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An immunohistochemical profile of giant cell carcinoma of the larynx.

Gurbuz Y, Kose N, Aydin O, Ozturk M
Auris, nasus, larynx 2007 Sep;34(3):413-6

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Mammary gland development in early pubertal female macaques.

Wood CE, Hester JM, Cline JM
Toxicologic pathology 2007 Oct;35(6):795-805

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Cellular and molecular mechanisms of abnormal calcification following ischemia-reperfusion injury in human liver transplantation.

Kalantari F, Miao D, Emadali A, Tzimas GN, Goltzman D, Vali H, Chevet E, Auguste P
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2007 Mar;20(3):357-66

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Analysis of keratin polypeptides 8 and 19 variants in inflammatory bowel disease.

Tao GZ, Strnad P, Zhou Q, Kamal A, Zhang L, Madani ND, Kugathasan S, Brant SR, Cho JH, Omary MB, Duerr RH
Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association 2007 Jul;5(7):857-64

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Blocking Wnt/LRP5 signaling by a soluble receptor modulates the epithelial to mesenchymal transition and suppresses met and metalloproteinases in osteosarcoma Saos-2 cells.

Guo Y, Zi X, Koontz Z, Kim A, Xie J, Gorlick R, Holcombe RF, Hoang BH
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2007 Jul;25(7):964-71

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Identification of 14-3-3sigma as a contributor to drug resistance in human breast cancer cells using functional proteomic analysis.

Liu Y, Liu H, Han B, Zhang JT
Cancer research 2006 Mar 15;66(6):3248-55

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Bispecific and human disease-related anti-keratin rabbit monoclonal antibodies.

Tao GZ, Nakamichi I, Ku NO, Wang J, Frolkis M, Gong X, Zhu W, Pytela R, Omary MB
Experimental cell research 2006 Feb 15;312(4):411-22

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Protein phosphatase-2A associates with and dephosphorylates keratin 8 after hyposmotic stress in a site- and cell-specific manner.

Tao GZ, Toivola DM, Zhou Q, Strnad P, Xu B, Michie SA, Omary MB
Journal of cell science 2006 Apr 1;119(Pt 7):1425-32

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Expression of Frzb/secreted Frizzled-related protein 3, a secreted Wnt antagonist, in human androgen-independent prostate cancer PC-3 cells suppresses tumor growth and cellular invasiveness.

Zi X, Guo Y, Simoneau AR, Hope C, Xie J, Holcombe RF, Hoang BH
Cancer research 2005 Nov 1;65(21):9762-70

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Studying simple epithelial keratins in cells and tissues.

Ku NO, Toivola DM, Zhou Q, Tao GZ, Zhong B, Omary MB
Methods in cell biology 2004;78:489-517

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Cytokeratin expression in lichen amyloidosus and macular amyloidosis.

Apaydin R, Gürbüz Y, Bayramgürler D, Müezzinoglu B, Bilen N
Journal of the European Academy of Dermatology and Venereology : JEADV 2004 May;18(3):305-9

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Keratin 8 and 18 hyperphosphorylation is a marker of progression of human liver disease.

Toivola DM, Ku NO, Resurreccion EZ, Nelson DR, Wright TL, Omary MB
Hepatology (Baltimore, Md.) 2004 Aug;40(2):459-66

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Keratin mutation in transgenic mice predisposes to Fas but not TNF-induced apoptosis and massive liver injury.

Ku NO, Soetikno RM, Omary MB
Hepatology (Baltimore, Md.) 2003 May;37(5):1006-14

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Keratin 8 mutations in patients with cryptogenic liver disease.

Ku NO, Gish R, Wright TL, Omary MB
The New England journal of medicine 2001 May 24;344(21):1580-7

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Cytokeratin 18 Monoclonal Antibody (DC10)(Product # MA1-39483) and a 48kDa band corresponding to Cytokeratin 18 was observed across the cell lines tested except IMR-32 and A-375. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), A549 (Lane 2), HeLa (Lane 3), Caco-2 (Lane 4), IMR-32 (Lane 5)and A-375 (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Cytokeratin 18 was performed using 70% confluent log phase Caco-2 cells. The cells were fixed with ice-cold acetone at 4°C for 5 minutes and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Cytokeratin 18 Monoclonal Antibody (DC10) (Product # MA1-39483) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 555 (Product # A32727), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415,1:300 dilution). Panel d represents the merged image showing cytoskeletal localization. Panel e represents A-375 cells lacking cytoskeletal staining. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Cytokeratin 18 using a monoclonal antibody (Product # MA1-39483).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 1 Cellular location of angiotensin-converting enzyme 2 (ACE-2) in human nasal and tracheal biopsies. a-d) Confocal image of ACE-2 (red) and Krt18 (green) immunostaining in the olfactory neuroepithelium from healthy control (a and c) and chronic rhinosinusitis (CRS) patient (b and d). The three-dimensional image (a) shows that the ACE-2 is localised to the apical surface of Krt18-positive sustentacular cells in the olfactory epithelium. Confocal images were obtained under Z stack mode which covered 8 um in depth. The boxed area in panel (a and b) was highlighted in (c and d), respectively. e and f) The location of ACE-2- and DCX-positive immature (e) or PGP9.5-positive mature (f) olfactory sensory neurons in control. g) Confocal image verified the apical expression pattern of ACE-2 by co-staining of Goat anti-ACE-2 and another Rabbit anti-ACE-2 antibody (clone SN0754). The boxed area in (g) was highlighted in right panels. h) Quantification of ACE-2-positive cells per mm olfactory epithelium (OE). At least three images were collected from each specimen (four controls and nine CRS biopsies) using 40xobjectives under the Z stack scan mode at same depth. i and j) Expression of ACE-2 in glands (i) and nasal respiratory epithelium (j). k) No detectable signal in Goat IgG isotype control. l) Quantification of ACE-2-positive cells per mm epithelium. The positive cells in seven tracheal biopsies and 13 nasal specimen that contained both respiratory and olfactory epithelium were