MA5-12104

antibody from Invitrogen Antibodies

Targeting: KRT18

Provider product page for MA5-12104

Western blot

Immunocytochemistry

Immunohistochemistry

Flow cytometry

Other assay

Antibody data

Antibody Data
Antigen structure
References [46]
Comments [0]
Validations
Immunocytochemistry [1]
Flow cytometry [1]
Other assay [8]

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Product number: MA5-12104 - Provider product page
Provider: Invitrogen Antibodies
Product name: Cytokeratin 18 Monoclonal Antibody (DC10)
Antibody type: Monoclonal
Antigen: Other
Description: MA5-12104 targets Cytokeratin 18 in IF, IHC (P), FACS, and WB applications and shows reactivity with Human samples. The MA5-12104 immunogen is human breast cancer PMC 42 cells.
Reactivity: Human, Mouse
Host: Mouse
Isotype: IgG
Antibody clone number: DC10
Vial size: 500 µL
Concentration: 0.2 mg/mL
Storage: 4° C

KRT18 protein structure - MA5-12104 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 2 Stained image of a cross-section of the tubular liver tissue perpendicular to the main blood vessel. ( a ) hematoxylin and eosin (HE) staining image. ( b ) immunohistochemistry (IHC) image (CD31). Asterisks indicate the main blood vessels. Blue arrowheads indicate capillaries. Red arrowheads indicate branches from main vessels to capillaries. ( c ) Immunofluorescence image (CK18 and CD31). ( d ) Schematic image of the tubular liver tissue.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Primary bEEC culture and identification. (A) Morphological observation of bEECs by ordinary optical microscopy (magnification, x200). (B) Primary bEECs were positive for the epithelial-specific marker CK-18 (red). (C) Nuclei were stained with DAPI (blue). (D) Merge of CK-18 and DAPI (magnification, x400). (E) The CK-18 + cells were counted using ImageJ. bEEC, bovine endometrial epithelial cell; CK-18, cytokeratin-18; IF, immunofluorescence.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 5. DSP -kd disrupts cell-cell junctions and cytoskeletal organization. CRISPRi-iAT2s were transduced with DSP gRNA and treated without (-dox) or with (+dox) doxycycline. ( A ) Immunofluorescence staining of desmoplakin (DSP). DSP, red; nuclei, blue; scale bar, 10 mum. ( B ) Flow cytometry analysis of DSP expression, represented by mean fluorescence intensity (MFI). n = 3 experimental replicates of independent wells of a differentiation; error bars represent SD. Statistical significance was determined by unpaired, two-tailed Student's t test; *** P < 0.001. ( C ) Transmission electron microscopy images showing tight junctions (TJ) and desmosomes (De); scale bar, 200 nm. ( D ) Violin plots showing module scores for KEGG (Kyoto Encyclopedia of Genes and Genomes) tight junction, cell adhesion molecules, and gap junction. ( E ) Dot plots showing expression of genes associated with desmosome, ( F ) adherens junction, and ( G ) TJ. Genes that were not expressed by iAT2s were excluded from dot plots. ( H ) Immunofluorescence staining of claudin-4, ZO-1, and E-cadherin. ( I ) Immunofluorescence staining of keratin-18 and alpha-tubulin. Arrows indicate disorganized filaments or tubules. Protein of interest, red; nuclei, blue; scale bar, 10 mum.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 3. GRHL3 is required to activate SE identity genes through opening their chromatin accessibilities. ( A ) Wild-type (WT) and GRHL3 -knockout hESCs during SE differentiation. Top: Phase contrast images. Bottom: Immunofluorescence staining of GRHL3 (green) and KRT18 (red). Scale bars, 100 mum. ( B ) Quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis of representative genes in wild-type and GRHL3 -knockout hESCs after 2 days of differentiation. qRT-PCR values were normalized to the values in wild-type group. Values are presented as means +- SD ( n = 3 biological replicates; * P < 0.05; ** P < 0.01; *** P < 0.001; t test). ( C ) Scatterplot of differential accessibility in wild-type versus GRHL3 -knockout (KO) hESCs after 2 days of differentiation. Sites identified as significantly differentially bound [log 2 fold change > 1 or < -1 and false discovery rate (FDR) < 0.05] are shown in color (red, peaks increased; blue, peaks decreased). ( D ) Metaplots of average ATAC-seq density around the SE identity genes in wild-type and GRHL3 -knockout hESCs after 2 days of differentiation. ( E ) Gene set enrichment analysis of the SE identity gene set in the gene expression matrix of wild-type and GRHL3 -knockout hESCs after 2 days of differentiation. NES, normalized enrichment score. ( F ) Enrichment of transcription factor motifs identified by HOMER at GRHL3 peaks. ( G ) Heatmaps of the binding signals of TFAP2C and GRHL2 at the center of GRHL3 peaks. ( H

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 4. GRHL3 induces SE commitment. ( A ) Schematic diagram of establishment of a TetO-GRHL3 doxycycline-inducible expression system in hESC. Cells were induced with doxycycline for 7 days. TRE, tetracycline response element; rtTA, reverse tetracycline transactivator. ( B ) Immunofluorescence staining of GRHL3 (green) and KRT18 (red) in TetO-GRHL3 + and TetO-GRHL3 - cells. Left: Phase contrast images. Scale bars, 100 mum. ( C ) qRT-PCR analysis of representative genes in TetO-GRHL3 + and TetO-GRHL3 - cells. qRT-PCR values were normalized to the values in TetO-GRHL3 - cells. Values are presented as means +- SD ( n = 3 biological replicates; ** P < 0.01; *** P < 0.001; t test). ( D ) Heatmap of germ layer-specific gene expression levels in TetO-GRHL3 + and TetO-GRHL3 - cells. ( E ) PCA of RNA-seq data of TetO-GRHL3 + cells and hESCs at each time points during SE differentiation. ( F ) Genome browser tracks comparing ATAC-seq signal at KRT8/18, KRT7, and TFAP2C loci in TetO-GRHL3 + and TetO-GRHL3 - cells. ( G ) Left: Scatterplot of differential accessibility in TetO-GRHL3 + versus TetO-GRHL3 - cells. Sites identified as significantly differentially bound are shown in color (red, peaks increased; blue, peaks decreased). Right: Transcription factor motif enrichment in the regions of differential accessibility in TetO-GRHL3 + versus TetO-GRHL3 - cells. TEAD, TEA domain transcription factor.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 Inducible EGFP-expressing and CTEN-expressing RWPE-1 cells perform similar phenotypes in the absence of doxycycline in 3D culture. Inducible EGFP-expressing (aON-EGFP) or CTEN-expressing (aON-CTEN) RWPE-1 cells were incubated in 3D culture system for 9 days without doxycycline induction. ( a ) The appearance of acinar structures was observed and classified to three types (normal, notched and deformed). The numbers of the three-type acini in each group were counted and presented as a percentage of the whole (upper panel). Data were expressed as mean +- SD of three independent experiments. More than 50 acini were evaluated within each sample (Student's t test; no significant difference between aON-EGFP and aON-CTEN in each phenotype). The representative images of acinar structure were also shown in the lower panel. Scale bar: 100 mum. ( b ) aON-EGFP and aON-CTEN RWPE-1 cells grown in 3D culture were collected at the indicated time and the protein levels were examined by Western analyses using the indicated antibodies. alpha-tubulin was used as a loading control.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 10 Cytoskeletal filaments decorate the CCV. HeLa cells were infected with C. burnetii at an MOI of 50. At 72 h post-infection, cells were fixed and stained with Phallotoxin-647 for actin, or with tubulin- and cytokeratin 18-specific antibodies by indirect immunofluorescence (green). Nuclei and bacterial DNA were stained with DAPI (blue). Cells were visualized using LSM. N: Nucleus. CCV: C. burnetii -containing vacuole.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 11 Cytoskeletal filaments are not modified by AnkF expression. HeLa cells were transiently transfected with pGFP alone or with pEGFP-AnkF (green). 24 h post-transfection, cells were fixed and stained with Phalloidin-Alexa647 for actin, or with tubulin- and cytokeratin 18-specific antibodies by indirect immunofluorescence (red). Nuclei and bacterial DNA were stained with DAPI (blue). Cells were visualized using LSM. Representative images from two independent experiments are shown.