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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-28279 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cytokeratin 18 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, H1299, HepG2.
- Concentration
- 0.54 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Cytokeratin 18 Polyclonal Antibody (Product # PA5-28279). Sample (30 µg of whole cell lysate). A: H1299. B: Hep G2. 7.5% SDS PAGE. Cytokeratin 18 Polyclonal Antibody (Product # PA5-28279) diluted at 1:3,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Cytokeratin 18 in paraformaldehyde-fixed HeLa cells using a Cytokeratin 18 polyclonal antibody (Product # PA5-28279) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cytokeratin 18 Polyclonal Antibody detects Cytokeratin 18 protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Cytokeratin 18 protein stained by Cytokeratin 18 Polyclonal Antibody (Product # PA5-28279) diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human endo mitral ovarian cancer, using KRT18 (Product # PA5-28279) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
