PA5-29609

antibody from Invitrogen Antibodies

Targeting: KRT18

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29609

Provider

Invitrogen Antibodies

Product name

Cytokeratin 18 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A431, H1299, HeLa, HepG2, Molt-4, Raji.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), HeLa (Lane 2), A-431 (Lane 3), Caco-2 (Lane 4), Caco-2 treated with IL-6 (100ng/ml for 24hr) (Lane 5) and A-375 (Lane 6). The blot was probed with Anti-Cytokeratin 18 Polyclonal Antibody (Product # PA5-29609, 1:4000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 48 kDa band corresponding to Cytokeratin 18 was observed across the cell lines tested. Also, the protein expression increased in Caco-2 cell line upon IL-6 treatment and was not detected in A-375 which is reported to be negative for Cytokeratin 18.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Cytokeratin 18 Polyclonal Antibody (Product # PA5-29609). Sample (30 µg of whole cell lysate). Lane A: Raji. 10% SDS PAGE. Cytokeratin 18 Polyclonal Antibody (Product # PA5-29609) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Cytokeratin 18 in paraformaldehyde-fixed HeLa cells using a Cytokeratin 18 polyclonal antibody (Product # PA5-29609) at a 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of Cytokeratin 18 was performed in paraffin-embedded mouse urinary bladder tissue using Cytokeratin 18 Polyclonal Antibody (Product # PA5-29609) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded H661 xenograft, using Cytokeratin 18 (Product # PA5-29609) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.