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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [17]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MA5-14088 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cytokeratin 8/18 Monoclonal Antibody (5D3)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-14088 targets Cytokeratin 8/18 in immunohistochemistry (paraffin) applications and shows reactivity with Human samples.
- Antibody clone number
- 5D3
- Concentration
- Conc. Not Determined
Submitted references Urinary Tract Tumor Organoids Reveal Eminent Differences in Drug Sensitivities When Compared to 2-Dimensional Culture Systems.
A 2-Step Laemmli and Antigen Retrieval Method Improves Immunodetection.
Infection of Human Retinal Pigment Epithelium with Chlamydia trachomatis.
A reference image-based method for optimization of clinical immunohistochemistry.
Pancreatic mucinous cystic tumor in Turner syndrome: How a tumor bends to a genetic disease.
Primary intracranial Ewing sarcoma with an unusually aggressive course: a case report and review of the literature.
CD49f-based selection of circulating tumor cells (CTCs) improves detection across breast cancer subtypes.
Sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression: report of a case and literature review.
Histological assessment of PAXgene tissue fixation and stabilization reagents.
Isolation and culture of adult human liver progenitor cells: in vitro differentiation to hepatocyte-like cells.
Primary sellar neuroblastoma presenting with syndrome of inappropriate secretion of anti-diuretic hormone.
Parietal epithelia cells in the urine as a marker of disease activity in glomerular diseases.
An immunohistochemical profile of giant cell carcinoma of the larynx.
Isolation, characterization, and differentiation to hepatocyte-like cells of nonparenchymal epithelial cells from adult human liver.
Breast cancer in an MSH2 gene mutation carrier.
An unusual presentation of Helicobacter pylori infection: so-called "Russell body gastritis".
Cytokeratin expression in lichen amyloidosus and macular amyloidosis.
Wei Y, Amend B, Todenhöfer T, Lipke N, Aicher WK, Fend F, Stenzl A, Harland N
International journal of molecular sciences 2022 Jun 4;23(11)
International journal of molecular sciences 2022 Jun 4;23(11)
A 2-Step Laemmli and Antigen Retrieval Method Improves Immunodetection.
Scalia CR, Gendusa R, Cattoretti G
Applied immunohistochemistry & molecular morphology : AIMM 2016 Jul;24(6):436-46
Applied immunohistochemistry & molecular morphology : AIMM 2016 Jul;24(6):436-46
Infection of Human Retinal Pigment Epithelium with Chlamydia trachomatis.
Boiko E, Maltsev D, Savicheva A, Shalepo K, Khusnutdinova T, Pozniak A, Kvetnoi I, Polyakova V, Suetov A
PloS one 2015;10(11):e0141754
PloS one 2015;10(11):e0141754
A reference image-based method for optimization of clinical immunohistochemistry.
Kap M, Lam KH, Ewing-Graham P, Riegman P
Histopathology 2015 Aug;67(2):193-205
Histopathology 2015 Aug;67(2):193-205
Pancreatic mucinous cystic tumor in Turner syndrome: How a tumor bends to a genetic disease.
Pizzi M, Pennelli G, Merante-Boschin I, Fassan M, Pelizzo MR, Rugge M
International journal of surgery case reports 2013;4(11):1028-31
International journal of surgery case reports 2013;4(11):1028-31
Primary intracranial Ewing sarcoma with an unusually aggressive course: a case report and review of the literature.
Tanboon J, Sitthinamsuwan B, Paruang T, Marrano P, Thorner PS
Neuropathology : official journal of the Japanese Society of Neuropathology 2012 Jun;32(3):293-300
Neuropathology : official journal of the Japanese Society of Neuropathology 2012 Jun;32(3):293-300
CD49f-based selection of circulating tumor cells (CTCs) improves detection across breast cancer subtypes.
Mostert B, Kraan J, Sieuwerts AM, van der Spoel P, Bolt-de Vries J, Prager-van der Smissen WJ, Smid M, Timmermans AM, Martens JW, Gratama JW, Foekens JA, Sleijfer S
Cancer letters 2012 Jun 1;319(1):49-55
Cancer letters 2012 Jun 1;319(1):49-55
Sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression: report of a case and literature review.
Yin WH, Li J, Chan JK
Diagnostic pathology 2012 Apr 12;7:39
Diagnostic pathology 2012 Apr 12;7:39
Histological assessment of PAXgene tissue fixation and stabilization reagents.
Kap M, Smedts F, Oosterhuis W, Winther R, Christensen N, Reischauer B, Viertler C, Groelz D, Becker KF, Zatloukal K, Langer R, Slotta-Huspenina J, Bodo K, de Jong B, Oelmuller U, Riegman P
PloS one 2011;6(11):e27704
PloS one 2011;6(11):e27704
Isolation and culture of adult human liver progenitor cells: in vitro differentiation to hepatocyte-like cells.
Gerbal-Chaloin S, Duret C, Raulet E, Navarro F, Blanc P, Ramos J, Maurel P, Daujat-Chavanieu M
Methods in molecular biology (Clifton, N.J.) 2010;640:247-60
Methods in molecular biology (Clifton, N.J.) 2010;640:247-60
Primary sellar neuroblastoma presenting with syndrome of inappropriate secretion of anti-diuretic hormone.
Radotra B, Apostolopoulos V, Sandison A, Hatfield EC, Mendoza N, Moss J, Mehta A, Glaser M, Meeran K, Roncaroli F
Endocrine pathology 2010 Dec;21(4):266-73
Endocrine pathology 2010 Dec;21(4):266-73
Parietal epithelia cells in the urine as a marker of disease activity in glomerular diseases.
Achenbach J, Mengel M, Tossidou I, Peters I, Park JK, Haubitz M, Ehrich JH, Haller H, Schiffer M
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2008 Oct;23(10):3138-45
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2008 Oct;23(10):3138-45
An immunohistochemical profile of giant cell carcinoma of the larynx.
Gurbuz Y, Kose N, Aydin O, Ozturk M
Auris, nasus, larynx 2007 Sep;34(3):413-6
Auris, nasus, larynx 2007 Sep;34(3):413-6
Isolation, characterization, and differentiation to hepatocyte-like cells of nonparenchymal epithelial cells from adult human liver.
Duret C, Gerbal-Chaloin S, Ramos J, Fabre JM, Jacquet E, Navarro F, Blanc P, Sa-Cunha A, Maurel P, Daujat-Chavanieu M
Stem cells (Dayton, Ohio) 2007 Jul;25(7):1779-90
Stem cells (Dayton, Ohio) 2007 Jul;25(7):1779-90
Breast cancer in an MSH2 gene mutation carrier.
Westenend PJ, Schütte R, Hoogmans MM, Wagner A, Dinjens WN
Human pathology 2005 Dec;36(12):1322-6
Human pathology 2005 Dec;36(12):1322-6
An unusual presentation of Helicobacter pylori infection: so-called "Russell body gastritis".
Ensari A, Savas B, Okcu Heper A, Kuzu I, Idilman R
Virchows Archiv : an international journal of pathology 2005 Apr;446(4):463-6
Virchows Archiv : an international journal of pathology 2005 Apr;446(4):463-6
Cytokeratin expression in lichen amyloidosus and macular amyloidosis.
Apaydin R, Gürbüz Y, Bayramgürler D, Müezzinoglu B, Bilen N
Journal of the European Academy of Dermatology and Venereology : JEADV 2004 May;18(3):305-9
Journal of the European Academy of Dermatology and Venereology : JEADV 2004 May;18(3):305-9
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Cytokeratin 8, 18 was performed using 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin 8, 18 (5D3) Mouse Monoclonal Antibody (Product # MA5-14088) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human skin stained with Keratin 8/18 antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of epithelial cells in the sweat glands.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of Cytokeratin 8, 18 was done on MCF7 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with Cytokeratin 8, 18 Mouse Monoclonal Antibody (MA5-14088, red histogram) or with mouse isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Rabbit Anti-Mouse Secondary Antibody (A11059) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10, 000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.
