NBP2-24787
antibody from Novus Biologicals
Targeting: TLR5
FLJ10052, MGC126430, MGC126431, SLEB1, TIL3
Antibody data
- Antibody Data
- Antigen structure
- References [36]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Flow cytometry [3]
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Validation data
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- Product number
- NBP2-24787 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal TLR5 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Canine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Elevated TLR5 expression in vivo and loss of NF-κΒ activation via TLR5 in vitro detected in HPV-negative oropharyngeal squamous cell carcinoma.
Expression of toll-like receptors in non-endemic nasopharyngeal carcinoma.
TLR5 and TLR7 are differentially expressed in human papillomavirus-positive and negative base of tongue squamous cell carcinoma, and TLR7 may have an independent prognostic influence.
Evaluation of toll-like receptors as prognostic biomarkers in gastric cancer: high tissue TLR5 predicts a better outcome.
Hsp90 Inhibition Reduces TLR5 Surface Expression and NF-κB Activation in Human Myeloid Leukemia THP-1 Cells.
Treponema denticola chymotrypsin-like protease as associated with HPV-negative oropharyngeal squamous cell carcinoma.
Substance P represents a novel first-line defense mechanism in the nose.
CXCR1 Regulates Pulmonary Anti-Pseudomonas Host Defense.
Nuclear localization of Toll-like receptor 5 in Barrett's esophagus and esophageal adenocarcinoma is associated with metastatic behavior.
Pellino-1 confers chemoresistance in lung cancer cells by upregulating cIAP2 through Lys63-mediated polyubiquitination.
The Expression of Toll-like Receptors in Normal Human and Murine Gastrointestinal Organs and the Effect of Microbiome and Cancer.
Expression of toll-like receptors in HPV-positive and HPV-negative oropharyngeal squamous cell carcinoma--an in vivo and in vitro study.
Predictive role of Toll-like receptors 2, 4, and 9 in oral tongue squamous cell carcinoma.
Expression of Toll-like receptors in nasal epithelium in allergic rhinitis.
Exhaustive exercise increases the TNF-α production in response to flagellin via the upregulation of toll-like receptor 5 in the large intestine in mice.
Increased Toll-like receptor 5 expression indicates esophageal columnar dysplasia.
Immunohistochemical detection of Helicobacter pylori without association of TLR5 expression in oral squamous cell carcinoma.
Identification of lineage relationships and novel markers of blood and skin human dendritic cells.
Toll-like receptor 5 (TLR5) expression is a novel predictive marker for recurrence and survival in squamous cell carcinoma of the tongue.
Membrane-tethered MUC1 mucin is phosphorylated by epidermal growth factor receptor in airway epithelial cells and associates with TLR5 to inhibit recruitment of MyD88.
Toll-like receptor responses of normal human urothelial cells to bacterial flagellin and lipopolysaccharide.
Toll-like receptor-induced innate immune responses in non-parenchymal liver cells are cell type-specific.
Antigen-containing liposomes engrafted with flagellin-related peptides are effective vaccines that can induce potent antitumor immunity and immunotherapeutic effect.
Toll-like receptors 4 and 5 induce distinct types of vasculitis.
Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry.
The myeloid differentiation factor 88 (MyD88) is required for CD4+ T cell effector function in a murine model of inflammatory bowel disease.
The myeloid differentiation factor 88 (MyD88) is required for CD4+ T cell effector function in a murine model of inflammatory bowel disease.
Stimulation by TLR5 modulates osteoclast differentiation through STAT1/IFN-beta.
Stimulation by TLR5 modulates osteoclast differentiation through STAT1/IFN-beta.
Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils.
TGF-alpha regulates TLR expression and function on epidermal keratinocytes.
TGF-alpha regulates TLR expression and function on epidermal keratinocytes.
Inhibition of neutrophil apoptosis by TLR agonists in whole blood: involvement of the phosphoinositide 3-kinase/Akt and NF-kappaB signaling pathways, leading to increased levels of Mcl-1, A1, and phosphorylated Bad.
Inhibition of neutrophil apoptosis by TLR agonists in whole blood: involvement of the phosphoinositide 3-kinase/Akt and NF-kappaB signaling pathways, leading to increased levels of Mcl-1, A1, and phosphorylated Bad.
Human CD4+ T cells express TLR5 and its ligand flagellin enhances the suppressive capacity and expression of FOXP3 in CD4+CD25+ T regulatory cells.
Human CD4+ T cells express TLR5 and its ligand flagellin enhances the suppressive capacity and expression of FOXP3 in CD4+CD25+ T regulatory cells.
Kylmä AK, Tolvanen TA, Carpén T, Haglund C, Mäkitie A, Mattila PS, Grenman R, Jouhi L, Sorsa T, Lehtonen S, Hagström J
Experimental and molecular pathology 2020 Jun;114:104435
Experimental and molecular pathology 2020 Jun;114:104435
Expression of toll-like receptors in non-endemic nasopharyngeal carcinoma.
Ruuskanen M, Leivo I, Minn H, Vahlberg T, Haglund C, Hagström J, Irjala H
BMC cancer 2019 Jun 25;19(1):624
BMC cancer 2019 Jun 25;19(1):624
TLR5 and TLR7 are differentially expressed in human papillomavirus-positive and negative base of tongue squamous cell carcinoma, and TLR7 may have an independent prognostic influence.
Haeggblom L, Näsman A, Ramqvist T, Haglund C, Hagström J, Mäkitie A, Dalianis T
Acta oto-laryngologica 2019 Feb;139(2):206-210
Acta oto-laryngologica 2019 Feb;139(2):206-210
Evaluation of toll-like receptors as prognostic biomarkers in gastric cancer: high tissue TLR5 predicts a better outcome.
Kasurinen A, Hagström J, Laitinen A, Kokkola A, Böckelman C, Haglund C
Scientific reports 2019 Aug 29;9(1):12553
Scientific reports 2019 Aug 29;9(1):12553
Hsp90 Inhibition Reduces TLR5 Surface Expression and NF-κB Activation in Human Myeloid Leukemia THP-1 Cells.
Na BH, Hoang TX, Kim JY
BioMed research international 2018;2018:4319369
BioMed research international 2018;2018:4319369
Treponema denticola chymotrypsin-like protease as associated with HPV-negative oropharyngeal squamous cell carcinoma.
Kylmä AK, Jouhi L, Listyarifah D, Mohamed H, Mäkitie A, Remes SM, Haglund C, Atula T, Nieminen MT, Sorsa T, Hagström J
British journal of cancer 2018 Jul;119(1):89-95
British journal of cancer 2018 Jul;119(1):89-95
Substance P represents a novel first-line defense mechanism in the nose.
Larsson O, Tengroth L, Xu Y, Uddman R, Kumlien Georén S, Cardell LO
The Journal of allergy and clinical immunology 2018 Jan;141(1):128-136.e3
The Journal of allergy and clinical immunology 2018 Jan;141(1):128-136.e3
CXCR1 Regulates Pulmonary Anti-Pseudomonas Host Defense.
Carevic M, Öz H, Fuchs K, Laval J, Schroth C, Frey N, Hector A, Bilich T, Haug M, Schmidt A, Autenrieth SE, Bucher K, Beer-Hammer S, Gaggar A, Kneilling M, Benarafa C, Gao JL, Murphy PM, Schwarz S, Moepps B, Hartl D
Journal of innate immunity 2016;8(4):362-73
Journal of innate immunity 2016;8(4):362-73
Nuclear localization of Toll-like receptor 5 in Barrett's esophagus and esophageal adenocarcinoma is associated with metastatic behavior.
Helminen O, Huhta H, Leppänen J, Kauppila JH, Takala H, Lehenkari PP, Saarnio J, Karttunen TJ
Virchows Archiv : an international journal of pathology 2016 Oct;469(4):465-70
Virchows Archiv : an international journal of pathology 2016 Oct;469(4):465-70
Pellino-1 confers chemoresistance in lung cancer cells by upregulating cIAP2 through Lys63-mediated polyubiquitination.
Jeon YK, Kim CK, Koh J, Chung DH, Ha GH
Oncotarget 2016 Jul 5;7(27):41811-41824
Oncotarget 2016 Jul 5;7(27):41811-41824
The Expression of Toll-like Receptors in Normal Human and Murine Gastrointestinal Organs and the Effect of Microbiome and Cancer.
Huhta H, Helminen O, Kauppila JH, Salo T, Porvari K, Saarnio J, Lehenkari PP, Karttunen TJ
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2016 Aug;64(8):470-82
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2016 Aug;64(8):470-82
Expression of toll-like receptors in HPV-positive and HPV-negative oropharyngeal squamous cell carcinoma--an in vivo and in vitro study.
Jouhi L, Datta N, Renkonen S, Atula T, Mäkitie A, Haglund C, Ahmed A, Syrjänen S, Grénman R, Auvinen E, Lehtonen S, Hagström J
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2015 Sep;36(10):7755-64
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2015 Sep;36(10):7755-64
Predictive role of Toll-like receptors 2, 4, and 9 in oral tongue squamous cell carcinoma.
Mäkinen LK, Atula T, Häyry V, Jouhi L, Datta N, Lehtonen S, Ahmed A, Mäkitie AA, Haglund C, Hagström J
Oral oncology 2015 Jan;51(1):96-102
Oral oncology 2015 Jan;51(1):96-102
Expression of Toll-like receptors in nasal epithelium in allergic rhinitis.
Renkonen J, Toppila-Salmi S, Joenväärä S, Mattila P, Parviainen V, Hagström J, Haglund C, Lehtonen M, Renkonen R
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2015 Aug;123(8):716-25
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2015 Aug;123(8):716-25
Exhaustive exercise increases the TNF-α production in response to flagellin via the upregulation of toll-like receptor 5 in the large intestine in mice.
Uchida M, Oyanagi E, Kawanishi N, Iemitsu M, Miyachi M, Kremenik MJ, Onodera S, Yano H
Immunology letters 2014 Mar-Apr;158(1-2):151-8
Immunology letters 2014 Mar-Apr;158(1-2):151-8
Increased Toll-like receptor 5 expression indicates esophageal columnar dysplasia.
Helminen O, Huhta H, Takala H, Lehenkari PP, Saarnio J, Kauppila JH, Karttunen TJ
Virchows Archiv : an international journal of pathology 2014 Jan;464(1):11-8
Virchows Archiv : an international journal of pathology 2014 Jan;464(1):11-8
Immunohistochemical detection of Helicobacter pylori without association of TLR5 expression in oral squamous cell carcinoma.
Grimm M, Munz A, Exarchou A, Polligkeit J, Reinert S
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 2014 Jan;43(1):35-44
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 2014 Jan;43(1):35-44
Identification of lineage relationships and novel markers of blood and skin human dendritic cells.
Harman AN, Bye CR, Nasr N, Sandgren KJ, Kim M, Mercier SK, Botting RA, Lewin SR, Cunningham AL, Cameron PU
Journal of immunology (Baltimore, Md. : 1950) 2013 Jan 1;190(1):66-79
Journal of immunology (Baltimore, Md. : 1950) 2013 Jan 1;190(1):66-79
Toll-like receptor 5 (TLR5) expression is a novel predictive marker for recurrence and survival in squamous cell carcinoma of the tongue.
Kauppila JH, Mattila AE, Karttunen TJ, Salo T
British journal of cancer 2013 Feb 19;108(3):638-43
British journal of cancer 2013 Feb 19;108(3):638-43
Membrane-tethered MUC1 mucin is phosphorylated by epidermal growth factor receptor in airway epithelial cells and associates with TLR5 to inhibit recruitment of MyD88.
Kato K, Lillehoj EP, Park YS, Umehara T, Hoffman NE, Madesh M, Kim KC
Journal of immunology (Baltimore, Md. : 1950) 2012 Feb 15;188(4):2014-22
Journal of immunology (Baltimore, Md. : 1950) 2012 Feb 15;188(4):2014-22
Toll-like receptor responses of normal human urothelial cells to bacterial flagellin and lipopolysaccharide.
Smith NJ, Varley CL, Eardley I, Feather S, Trejdosiewicz LK, Southgate J
The Journal of urology 2011 Sep;186(3):1084-92
The Journal of urology 2011 Sep;186(3):1084-92
Toll-like receptor-induced innate immune responses in non-parenchymal liver cells are cell type-specific.
Wu J, Meng Z, Jiang M, Zhang E, Trippler M, Broering R, Bucchi A, Krux F, Dittmer U, Yang D, Roggendorf M, Gerken G, Lu M, Schlaak JF
Immunology 2010 Mar;129(3):363-74
Immunology 2010 Mar;129(3):363-74
Antigen-containing liposomes engrafted with flagellin-related peptides are effective vaccines that can induce potent antitumor immunity and immunotherapeutic effect.
Faham A, Altin JG
Journal of immunology (Baltimore, Md. : 1950) 2010 Aug 1;185(3):1744-54
Journal of immunology (Baltimore, Md. : 1950) 2010 Aug 1;185(3):1744-54
Toll-like receptors 4 and 5 induce distinct types of vasculitis.
Deng J, Ma-Krupa W, Gewirtz AT, Younge BR, Goronzy JJ, Weyand CM
Circulation research 2009 Feb 27;104(4):488-95
Circulation research 2009 Feb 27;104(4):488-95
Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry.
Burgener IA, Jungi TW
Veterinary immunology and immunopathology 2008 Jul 15;124(1-2):184-91
Veterinary immunology and immunopathology 2008 Jul 15;124(1-2):184-91
The myeloid differentiation factor 88 (MyD88) is required for CD4+ T cell effector function in a murine model of inflammatory bowel disease.
Fukata M, Breglio K, Chen A, Vamadevan AS, Goo T, Hsu D, Conduah D, Xu R, Abreu MT
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 1;180(3):1886-94
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 1;180(3):1886-94
The myeloid differentiation factor 88 (MyD88) is required for CD4+ T cell effector function in a murine model of inflammatory bowel disease.
Fukata M, Breglio K, Chen A, Vamadevan AS, Goo T, Hsu D, Conduah D, Xu R, Abreu MT
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 1;180(3):1886-94
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 1;180(3):1886-94
Stimulation by TLR5 modulates osteoclast differentiation through STAT1/IFN-beta.
Ha H, Lee JH, Kim HN, Kwak HB, Kim HM, Lee SE, Rhee JH, Kim HH, Lee ZH
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 1;180(3):1382-9
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 1;180(3):1382-9
Stimulation by TLR5 modulates osteoclast differentiation through STAT1/IFN-beta.
Ha H, Lee JH, Kim HN, Kwak HB, Kim HM, Lee SE, Rhee JH, Kim HH, Lee ZH
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 1;180(3):1382-9
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 1;180(3):1382-9
Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils.
Wong CK, Cheung PF, Ip WK, Lam CW
American journal of respiratory cell and molecular biology 2007 Jul;37(1):85-96
American journal of respiratory cell and molecular biology 2007 Jul;37(1):85-96
TGF-alpha regulates TLR expression and function on epidermal keratinocytes.
Miller LS, Sørensen OE, Liu PT, Jalian HR, Eshtiaghpour D, Behmanesh BE, Chung W, Starner TD, Kim J, Sieling PA, Ganz T, Modlin RL
Journal of immunology (Baltimore, Md. : 1950) 2005 May 15;174(10):6137-43
Journal of immunology (Baltimore, Md. : 1950) 2005 May 15;174(10):6137-43
TGF-alpha regulates TLR expression and function on epidermal keratinocytes.
Miller LS, Sørensen OE, Liu PT, Jalian HR, Eshtiaghpour D, Behmanesh BE, Chung W, Starner TD, Kim J, Sieling PA, Ganz T, Modlin RL
Journal of immunology (Baltimore, Md. : 1950) 2005 May 15;174(10):6137-43
Journal of immunology (Baltimore, Md. : 1950) 2005 May 15;174(10):6137-43
Inhibition of neutrophil apoptosis by TLR agonists in whole blood: involvement of the phosphoinositide 3-kinase/Akt and NF-kappaB signaling pathways, leading to increased levels of Mcl-1, A1, and phosphorylated Bad.
François S, El Benna J, Dang PM, Pedruzzi E, Gougerot-Pocidalo MA, Elbim C
Journal of immunology (Baltimore, Md. : 1950) 2005 Mar 15;174(6):3633-42
Journal of immunology (Baltimore, Md. : 1950) 2005 Mar 15;174(6):3633-42
Inhibition of neutrophil apoptosis by TLR agonists in whole blood: involvement of the phosphoinositide 3-kinase/Akt and NF-kappaB signaling pathways, leading to increased levels of Mcl-1, A1, and phosphorylated Bad.
François S, El Benna J, Dang PM, Pedruzzi E, Gougerot-Pocidalo MA, Elbim C
Journal of immunology (Baltimore, Md. : 1950) 2005 Mar 15;174(6):3633-42
Journal of immunology (Baltimore, Md. : 1950) 2005 Mar 15;174(6):3633-42
Human CD4+ T cells express TLR5 and its ligand flagellin enhances the suppressive capacity and expression of FOXP3 in CD4+CD25+ T regulatory cells.
Crellin NK, Garcia RV, Hadisfar O, Allan SE, Steiner TS, Levings MK
Journal of immunology (Baltimore, Md. : 1950) 2005 Dec 15;175(12):8051-9
Journal of immunology (Baltimore, Md. : 1950) 2005 Dec 15;175(12):8051-9
Human CD4+ T cells express TLR5 and its ligand flagellin enhances the suppressive capacity and expression of FOXP3 in CD4+CD25+ T regulatory cells.
Crellin NK, Garcia RV, Hadisfar O, Allan SE, Steiner TS, Levings MK
Journal of immunology (Baltimore, Md. : 1950) 2005 Dec 15;175(12):8051-9
Journal of immunology (Baltimore, Md. : 1950) 2005 Dec 15;175(12):8051-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TLR5 Antibody (19D759.2) [NBP2-24787] - BEAS-2B cells whole cell lysate. Image from verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TLR5 Antibody (19D759.2) [NBP2-24787] - Human skin stained with TLR5 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TLR5 Antibody (19D759.2) [NBP2-24787] - Analysis of FFPE tissue section of normal human skin using 5 ug/ml concentration of TLR5 antibody (clone 19D759.2). Intense membrane staining of TLR5 was observed specifically in the pigmented basel cells of epidermal layer [10X Magnification]
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TLR5 Antibody (19D759.2) [NBP2-24787] - Analysis of TLR5 in FFPE mouse brain tissue using TLR5 antibody at 5 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR5 Antibody (19D759.2) [NBP2-24787] - Intracellular flow analysis of TLR5 in human monocytes using 0.5 ug of TLR5 antibody. Shaded histogram represents cells without antibody; green represents isotype control; red represents TLR5 antibody. this antibody secondary antibody was used in this test.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR5 Antibody (19D759.2) [NBP2-24787] - Analysis using the PE conjugate of NBP2-24787. Staining of CD14-/CD83+/TLR5+ diffferentiated myeloid dendritic cells (mDCs). PBMCs were stimulated 5 days with GM-CSF+IL4 prior to the staining. The cells were stained for surface markers CD14 and CD83 FITC, and TLR5 PE. CD14- cells were gated and stained with PE-conjugated isotype control (mouse IgG2a), and FITC-conjugated CD83 (left). CD14- cells were gated and stained with 1 ug PE-conjugated TLR5 , and FITC-conjugated CD83 (right).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR5 Antibody (19D759.2) [NBP2-24787] - Analysis using the FITC conjugate of NBP2-24787. Staining of TLR5 in PBMCs using 0.5 ug/10^6 cells of this antibody. Shaded histogram represents cells without antibody; green represents isotype control; red represents anti-TLR5 antibody.